Cheng Yufei, Li Wei, Jin Tingting, Wu Sisi, Zhang Longhao
Core Facilities, West China Hospital of Sichuan University, Chengdu 610041, P. R. China.
Department of Medical Discipline Construction, West China hospital of Sichuan University, Chengdu 610041, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2023 Feb 25;40(1):180-184. doi: 10.7507/1001-5515.202210060.
This paper reviews the research progress on live-cell super-resolution fluorescence microscopy, discusses the current research status and hotspots in this field, and summarizes the technological application of super-resolution fluorescence microscopy for live-cell imaging. To date, this field has gained progress in numerous aspects. Specifically, the structured illumination microscopy, stimulated emission depletion microscopy, and the recently introduced minimal photon fluxes microscopy are the current research hotspots. According to the current progress in this field, future development trend is likely to be largely driven by artificial intelligence as well as advances in fluorescent probes and relevant labelling methods.
本文综述了活细胞超分辨率荧光显微镜的研究进展，讨论了该领域的研究现状和热点，并总结了超分辨率荧光显微镜在活细胞成像中的技术应用。迄今为止，该领域在诸多方面都取得了进展。具体而言，结构光照明显微镜、受激发射损耗显微镜以及最近推出的最小光子通量显微镜是当前的研究热点。根据该领域目前的进展，未来的发展趋势可能在很大程度上由人工智能以及荧光探针和相关标记方法的进步所驱动。
