Matsuura Kazunori, Hisamoto Koichi, Tanaka Tomoya, Sakamoto Ryota, Okazaki Mizuki, Inaba Hiroshi
Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
Centre for Research on Green Sustainable Chemistry, Tottori University, Tottori 680-8552, Japan.
ACS Org Inorg Au. 2021 Aug 10;1(2):60-67. doi: 10.1021/acsorginorgau.1c00013. eCollection 2021 Dec 1.
Gangliosides play pivotal biological roles in the animal cell membranes, and it is vital to develop fluorescent probes for imaging them. To date, various artificial receptors for ganglioside imaging have been developed; however, turn-on fluorescence imaging for gangliosides with high contrast has not been achieved. We developed a simple fluorescent probe on the basis of a dansyl triarginine peptide for turn-on ganglioside imaging on the liposome membrane. The probe bound to monosialyl gangliosides and other anionic lipids with association constants was 10 M, which enhanced from 6-fold to 7-fold the fluorescence intensity. Upon binding to monosialyl ganglioside-containing giant liposomes, the turn-on probe selectively enhanced the fluorescence intensity compared with the other anionic lipids. This simple peptide probe for turn-on fluorescence imaging of gangliosides would provide a novel molecular tool for chemical biology.
神经节苷脂在动物细胞膜中发挥着关键的生物学作用，开发用于对其进行成像的荧光探针至关重要。迄今为止，已开发出各种用于神经节苷脂成像的人工受体；然而，尚未实现对神经节苷脂进行具有高对比度的开启式荧光成像。我们基于丹磺酰三精氨酸肽开发了一种简单的荧光探针，用于在脂质体膜上对神经节苷脂进行开启式成像。该探针与单唾液酸神经节苷脂及其他阴离子脂质结合，缔合常数为10 M，荧光强度增强了6至7倍。与其他阴离子脂质相比，该开启式探针在与含单唾液酸神经节苷脂的巨型脂质体结合后，选择性地增强了荧光强度。这种用于神经节苷脂开启式荧光成像的简单肽探针将为化学生物学提供一种新型分子工具。
