College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University , Tianjin 300071, China.
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
Anal Chem. 2017 Jun 6;89(11):5874-5881. doi: 10.1021/acs.analchem.7b00199. Epub 2017 May 10.
Precise profiling of the sialic acid (SA) expression on the membrane of cancer cells is critical for early identification of cancers and assessment of cancer metastasis. However, the complex physiological environments often result in false positives with currently available imaging technologies. Herein, we have established a background-free surface-enhanced Raman scattering (SERS) imaging platform that allows high-precision profiling of SA expression in cancer cells and differentiation of clinically relevant cancer tissues with various metastasis degrees. Three-dimensional Raman imaging technique provided a deeper insight into visualizing the probe distribution and thus the SA expression at the single-cell level, without destructing the cells. This noninvasive, high-precision imaging technique could favor early diagnosis, staging, and monitoring therapeutic responses of cancers that are highly essential in clinical settings.
精准分析癌细胞表面唾液酸（SA）的表达对于癌症的早期诊断和转移评估至关重要。然而，目前可用的成像技术往往会受到复杂生理环境的影响，导致假阳性。在此，我们建立了一种无背景的表面增强拉曼散射（SERS）成像平台，可以高精度地分析癌细胞中 SA 的表达，并对具有不同转移程度的临床相关癌组织进行区分。三维拉曼成像技术提供了更深入的了解，能够在不破坏细胞的情况下，在单细胞水平上可视化探针分布和 SA 表达。这种非侵入性、高精度的成像技术有利于癌症的早期诊断、分期和治疗反应监测，在临床环境中具有重要意义。
