Baatrup G, Thiel S, Isager H, Svehag S E, Jensenius J C
Institute of Medical Microbiology, Odense University, Denmark.
Scand J Immunol. 1987 Oct;26(4):355-61. doi: 10.1111/j.1365-3083.1987.tb02267.x.
Evidence of the existence in human plasma of an activity analogous to that of bovine conglutinin is presented. The human plasma component was characterized antigenically and functionally. Human plasma was shown to agglutinate complement-coated erythrocytes in the presence of Ca2+, and this conglutination was inhibited by EDTA. The molecule also binds to complement-reacted solid phase IgG and to zymosan in the presence of Ca2+. The binding to complement is not inhibited by N-acetyl-D-mannosamine, but is inhibited by N-acetyl-D-glucosamine, as previously shown for bovine conglutinin. Antibodies raised against bovine conglutinin cross-react with the human protein. The plasma concentration of the conglutinin-like protein showed a high inter-individual variation between apparently healthy donors. Unlike bovine conglutinin, the human conglutinin activity could not be demonstrated in serum but only in plasma. The activity was more stable in plasma containing metal-ion chelators like EDTA and citrate than in heparin or hirudin plasma.
本文提供了人类血浆中存在与牛胶固素活性类似的活性的证据。对人血浆成分进行了抗原和功能特性分析。结果表明，人血浆在Ca2+存在下能凝集补体包被的红细胞，且这种凝集作用被EDTA抑制。该分子在Ca2+存在下还能与补体反应的固相IgG及酵母聚糖结合。与补体的结合不受N-乙酰-D-甘露糖胺抑制，但受N-乙酰-D-葡萄糖胺抑制，这与牛胶固素的情况一致。针对牛胶固素产生的抗体与人蛋白发生交叉反应。在明显健康的供体中，类胶固素蛋白的血浆浓度个体间差异很大。与牛胶固素不同，人胶固素活性在血清中无法检测到，仅在血浆中存在。在含有EDTA和柠檬酸盐等金属离子螯合剂的血浆中，该活性比在肝素或水蛭素血浆中更稳定。
