Friis P, Svehag S E, Andersen O, Gahrn-Hansen B, Leslie R G
Department of Medical Microbiology, Odense University, Denmark.
Immunology. 1991 Dec;74(4):680-4.
Conglutinin is a mammalian C-type lectin which shows anti-bacterial activity when tested in vivo and in vitro. This study concerns the effect of conglutinin on the respiratory burst of murine spleen cells, using a chemiluminescence assay for measurement of generated reactive oxygen metabolites. Conglutinin enhances, in a dose-dependent manner, the respiratory burst of spleen cells stimulated with serum-opsonized Escherichia coli. The enhancement was only demonstrable in the presence of a functional complement system. The conglutinin-mediated enhancement of the respiratory burst was inhibited in the presence of a N-acetyl-D-glucosamine, D-mannose and N-acetyl-D-mannosamine, monosaccharides reported to inhibit conglutinin-binding to zymosan and the complement factor iC3b. On the other hand, N-acetyl-D-galactosamine was non-inhibitory.
胶固素是一种哺乳动物C型凝集素，在体内和体外测试时均显示出抗菌活性。本研究利用化学发光测定法测量产生的活性氧代谢产物，探讨胶固素对小鼠脾细胞呼吸爆发的影响。胶固素以剂量依赖的方式增强血清调理的大肠杆菌刺激的脾细胞的呼吸爆发。这种增强仅在功能性补体系统存在时才得以证明。在存在N-乙酰-D-葡萄糖胺、D-甘露糖和N-乙酰-D-甘露糖胺的情况下，胶固素介导的呼吸爆发增强受到抑制，这些单糖据报道可抑制胶固素与酵母聚糖和补体因子iC3b的结合。另一方面，N-乙酰-D-半乳糖胺没有抑制作用。
