Haas J G, Riethmüller G, Ziegler-Heitbrock H W
Institute of Immunology, University of Munich, FRG.
Scand J Immunol. 1987 Oct;26(4):371-9. doi: 10.1111/j.1365-3083.1987.tb02269.x.
The CD4 molecule, which is known to play an important role in the susceptibility of T lymphocytes to infection by the human immunodeficiency virus (HIV), is also expressed in small amounts on the surface of monocytes. Since monocytes can also be infected by the virus, we investigated peripheral blood monocytes of patients with the acquired immunodeficiency syndrome (AIDS), AIDS-related complex (ARC), and HIV seropositive and seronegative haemophiliacs without symptoms for the expression of the CD4 molecule and for other functionally important surface molecules such as CD11 (C3bi receptor), transferrin receptor, Fc receptor, and the three major histocompatibility complex (MHC) class II antigens HLA-DP, HLA-DR, and HLA-DQ. With immunofluorescence staining and flow cytometry no difference was found between patients and controls for the expression of the CD4 molecule and for the other antigens as assessed by the percentage of positive staining and the specific fluorescence intensity in a double marker analysis. The percentage of CD4+ monocytes was found to be 59.2 +/- 14.4% for 16 patients with AIDS and 52.9 +/- 12.8% for 12 healthy controls. Similar to our results on phenotype, we found no significant difference with respect to the production of tumour necrosis factor (TNF), in that monocytes of AIDS and ARC patients showed an increase in TNF secretion after stimulation with LPS comparable to controls.
已知CD4分子在T淋巴细胞对人类免疫缺陷病毒（HIV）感染的易感性中起重要作用，它在单核细胞表面也有少量表达。由于单核细胞也可被该病毒感染，我们研究了获得性免疫缺陷综合征（AIDS）患者、艾滋病相关综合征（ARC）患者以及无症状的HIV血清阳性和血清阴性血友病患者的外周血单核细胞，以检测CD4分子以及其他功能重要的表面分子的表达情况，这些分子包括CD11（C3bi受体）、转铁蛋白受体、Fc受体以及三种主要的组织相容性复合体（MHC）II类抗原HLA-DP、HLA-DR和HLA-DQ。通过免疫荧光染色和流式细胞术，在双标记分析中，根据阳性染色百分比和特异性荧光强度评估，患者与对照之间在CD4分子及其他抗原的表达上未发现差异。16例AIDS患者的CD4 +单核细胞百分比为59.2±14.4%，12例健康对照为52.9±12.8%。与我们在表型方面的结果相似，我们发现肿瘤坏死因子（TNF）的产生没有显著差异，即AIDS和ARC患者的单核细胞在用脂多糖刺激后TNF分泌增加，与对照相当。
