Faculty of Veterinary Medicine, Department of Internal Medicine, Kastamonu University, Kastamonu, Türkiye.
Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Bingöl University, Bingöl, Türkiye.
Vet Clin Pathol. 2023 Jun;52(2):288-294. doi: 10.1111/vcp.13223. Epub 2023 Mar 1.
Accurate and rapid measurement of blood β-hydroxybutyrate (β-OHB) concentrations is critical to identify hyperketonemia in sheep.
The objective of this study was to test the performance of Freestyle Optium Neo H and TaiDoc-TD4235 point-of-care (POC) devices for measuring blood β-OHB concentrations in sheep.
Venous blood samples from 105 sheep were evaluated with Freestyle Optium Neo H and TaiDoc-TD4235 meters. A reference approach was the laboratory measurement of serum BHB concentrations using the Randox D-3 Hydroxybutyrate reagent kit. Data were analyzed using Passing- Bablok regression and Bland-Altman analysis. The diagnostic accuracy was assessed using serum β-OHB concentration at a cut-off value of 0.8 mmol/L.
Passing-Bablok regression analysis revealed an intercept of 0.066 (CI : 0.018-0.125) and slope of 1.242 (CI : 1.136-1.342) for Freestyle Optium Neo H. Intercept and slope values for the TaiDoc-TD4235 meter were 0.625 (CI : 0.539-0.724) and 1.265 (CI : 1.044-1.497), respectively. The Bland-Altman plot revealed a bias of 0.224 mmol/L and 0.737 mmol/L for Freestyle Optium Neo H and TaiDoc-TD4235 meters, respectively, compared with the reference method. Observed Total Error (TE ) was 56.7% for the Freestyle Optium Neo H and 168.7% for the Taidoc-TD4235. The sensitivity and specificity for the Freestyle Optium Neo H were 100% and 80.9%, respectively, at the threshold of 0.8 mmol/L. The Taidoc-TD4235 demonstrated a sensitivity of 93.3% and specificity of 8.9% at the threshold of 0.8 mmol/L.
Because of the large TE , the Freestyle Optium Neo H and TaiDoc-TD4235 meters should not be used to quantify blood β-OHB concentrations in sheep.
准确、快速地测量血 β-羟丁酸（β-OHB）浓度对于识别绵羊的高酮血症至关重要。
本研究旨在测试 Freestyle Optium Neo H 和 TaiDoc-TD4235 即时检测（POC）设备用于测量绵羊血 β-OHB 浓度的性能。
对 105 只绵羊的静脉血样本分别用 Freestyle Optium Neo H 和 TaiDoc-TD4235 测定仪进行检测。参考方法是使用 Randox D-3 Hydroxybutyrate 试剂试剂盒测量血清 BHB 浓度。采用 Passing-Bablok 回归和 Bland-Altman 分析对数据进行分析。使用血清β-OHB 浓度为 0.8mmol/L 的截断值评估诊断准确性。
Passing-Bablok 回归分析显示，Freestyle Optium Neo H 的截距为 0.066（CI：0.018-0.125），斜率为 1.242（CI：1.136-1.342）。TaiDoc-TD4235 仪的截距和斜率值分别为 0.625（CI：0.539-0.724）和 1.265（CI：1.044-1.497）。Bland-Altman 图显示，与参考方法相比，Freestyle Optium Neo H 和 TaiDoc-TD4235 仪的偏差分别为 0.224mmol/L 和 0.737mmol/L。Freestyle Optium Neo H 的观察总误差（TE）为 56.7%，TaiDoc-TD4235 的观察总误差为 168.7%。Freestyle Optium Neo H 在阈值为 0.8mmol/L 时的灵敏度和特异性分别为 100%和 80.9%。在阈值为 0.8mmol/L 时，TaiDoc-TD4235 的灵敏度为 93.3%，特异性为 8.9%。
由于 TE 较大，Freestyle Optium Neo H 和 TaiDoc-TD4235 仪不应用于定量绵羊血 β-OHB 浓度。
