评价 Freestyle Optium Neo H 和 TaiDoc-TD4235 即时检测血糖仪在绵羊血液β-羟丁酸浓度检测中的应用。

Evaluation of Freestyle Optium Neo H and TaiDoc-TD4235 point-of-care meters for measuring blood β-hydroxybutyrate concentration in sheep.

机构信息

Faculty of Veterinary Medicine, Department of Internal Medicine, Kastamonu University, Kastamonu, Türkiye.

Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Bingöl University, Bingöl, Türkiye.

出版信息

Vet Clin Pathol. 2023 Jun;52(2):288-294. doi: 10.1111/vcp.13223. Epub 2023 Mar 1.

DOI:10.1111/vcp.13223

PMID:36859602

Abstract

BACKGROUND

Accurate and rapid measurement of blood β-hydroxybutyrate (β-OHB) concentrations is critical to identify hyperketonemia in sheep.

OBJECTIVE

The objective of this study was to test the performance of Freestyle Optium Neo H and TaiDoc-TD4235 point-of-care (POC) devices for measuring blood β-OHB concentrations in sheep.

METHODS

Venous blood samples from 105 sheep were evaluated with Freestyle Optium Neo H and TaiDoc-TD4235 meters. A reference approach was the laboratory measurement of serum BHB concentrations using the Randox D-3 Hydroxybutyrate reagent kit. Data were analyzed using Passing- Bablok regression and Bland-Altman analysis. The diagnostic accuracy was assessed using serum β-OHB concentration at a cut-off value of 0.8 mmol/L.

RESULTS

Passing-Bablok regression analysis revealed an intercept of 0.066 (CI : 0.018-0.125) and slope of 1.242 (CI : 1.136-1.342) for Freestyle Optium Neo H. Intercept and slope values for the TaiDoc-TD4235 meter were 0.625 (CI : 0.539-0.724) and 1.265 (CI : 1.044-1.497), respectively. The Bland-Altman plot revealed a bias of 0.224 mmol/L and 0.737 mmol/L for Freestyle Optium Neo H and TaiDoc-TD4235 meters, respectively, compared with the reference method. Observed Total Error (TE ) was 56.7% for the Freestyle Optium Neo H and 168.7% for the Taidoc-TD4235. The sensitivity and specificity for the Freestyle Optium Neo H were 100% and 80.9%, respectively, at the threshold of 0.8 mmol/L. The Taidoc-TD4235 demonstrated a sensitivity of 93.3% and specificity of 8.9% at the threshold of 0.8 mmol/L.

CONCLUSIONS

Because of the large TE , the Freestyle Optium Neo H and TaiDoc-TD4235 meters should not be used to quantify blood β-OHB concentrations in sheep.

摘要

背景

准确、快速地测量血 β-羟丁酸（β-OHB）浓度对于识别绵羊的高酮血症至关重要。

目的

本研究旨在测试 Freestyle Optium Neo H 和 TaiDoc-TD4235 即时检测（POC）设备用于测量绵羊血 β-OHB 浓度的性能。

方法

对 105 只绵羊的静脉血样本分别用 Freestyle Optium Neo H 和 TaiDoc-TD4235 测定仪进行检测。参考方法是使用 Randox D-3 Hydroxybutyrate 试剂试剂盒测量血清 BHB 浓度。采用 Passing-Bablok 回归和 Bland-Altman 分析对数据进行分析。使用血清β-OHB 浓度为 0.8mmol/L 的截断值评估诊断准确性。

结果

Passing-Bablok 回归分析显示，Freestyle Optium Neo H 的截距为 0.066（CI：0.018-0.125），斜率为 1.242（CI：1.136-1.342）。TaiDoc-TD4235 仪的截距和斜率值分别为 0.625（CI：0.539-0.724）和 1.265（CI：1.044-1.497）。Bland-Altman 图显示，与参考方法相比，Freestyle Optium Neo H 和 TaiDoc-TD4235 仪的偏差分别为 0.224mmol/L 和 0.737mmol/L。Freestyle Optium Neo H 的观察总误差（TE）为 56.7%，TaiDoc-TD4235 的观察总误差为 168.7%。Freestyle Optium Neo H 在阈值为 0.8mmol/L 时的灵敏度和特异性分别为 100%和 80.9%。在阈值为 0.8mmol/L 时，TaiDoc-TD4235 的灵敏度为 93.3%，特异性为 8.9%。