Uztimür Murat, Ünal Cennet Nur, Deniz Abdülkerim, Pekmezci Aytaç
Faculty of Veterinary Medicine, Department of Internal Medicine, Bingöl University, Bingol, Turkey.
Free Researcher for Clinical Biochemistry, İstanbul, Turkey.
Vet Med Sci. 2025 Jan;11(1):e70159. doi: 10.1002/vms3.70159.
There is a lack of data on the validation and diagnostic performance of the Freestyle Optium Neo-H (Freestyle) and Centrivet GK (Centrivet) devices for the diagnosis of hypoglycaemia, hyperglycaemia and hyperketonaemia in goats.
The aim of the present study was to validate the Freestyle and Centrivet for the analysis of whole blood beta-hydroxybutyric acid (BHBA) and to validate the Freestyle for the analysis of whole blood glucose concentrations using the reference method (RM) in goat blood collected from the jugular and ear veins.
Venous blood samples were utilised to assess glucose and BHBA concentrations using the Freestyle, Centrivet and RM. The cut-off point of BHBA was ≥ 0.8 mmol/L for hyperketonaemia. A total of 198 paired blood samples (vena jugularis and ear vein) were collected from 99 hair goats. The cut-off point for hypoglycaemia diagnosis was < 49 mg/dL.
There were proportional but no constant errors between RM and Freestyle and Centrivet for BHBA, and both proportional and constant errors were observed for glucose analysis. The mean bias for BHBA analysis was 0.14 and 0.06 mmol/L (Freestyle-RM) 0.51 and 0.16 mmol/L (Centrivet-RM) for jugular and ear veins, respectively. The mean bias for blood glucose analysis was 0.0 and 5.6 mg/L between Freestyle and RM in the jugular and ear veins, respectively. The sensitivity (Centrivet: 50%-61.3%; Freestyle: 93.6%-75.8%) and specificity (Centrivet GK: 75.7%-73%; Freestyle: 37.8%-70.3%) were determined in jugular and ear vein blood for hyperketonaemia diagnostics, respectively. The AUC of Freestyle was 0.89 and 0.95 in the jugular and ear vein for hypoglycaemia, respectively. The sensitivity of Freestyle was 60.3% and 96.8% in the jugular and ear vein for hypoglycaemia. The specificity of Freestyle was 100.0% and 76.7% for hypoglycaemia in jugular and ear veins, respectively.
Freestyle demonstrated acceptable diagnostic performance for hypoglycaemia in ear veins, but neither Freestyle nor Centrivet showed sufficient diagnostic performance for hyperketonaemia. Both analysers were not interchangeable with RM in BHBA and glucose analysis.
目前缺乏关于Freestyle Optium Neo-H（Freestyle）和Centrivet GK（Centrivet）设备在山羊低血糖、高血糖和高酮血症诊断方面的验证及诊断性能的数据。
本研究旨在验证Freestyle和Centrivet用于分析全血β-羟基丁酸（BHBA）的性能，并使用参考方法（RM）验证Freestyle用于分析从颈静脉和耳静脉采集的山羊血液中全血葡萄糖浓度的性能。
利用静脉血样本，通过Freestyle、Centrivet和RM评估葡萄糖和BHBA浓度。高酮血症时BHBA的截断点为≥0.8 mmol/L。从99只毛用山羊中总共采集了198对血液样本（颈静脉和耳静脉）。低血糖诊断的截断点为<49 mg/dL。
RM与Freestyle和Centrivet在BHBA分析上存在比例误差但无恒定误差，而在葡萄糖分析中同时观察到比例误差和恒定误差。颈静脉和耳静脉中，Freestyle - RM和Centrivet - RM在BHBA分析中的平均偏差分别为0.14和0.06 mmol/L以及0.51和0.16 mmol/L。颈静脉和耳静脉中，Freestyle与RM在血糖分析中的平均偏差分别为0.0和5.6 mg/L。分别在颈静脉和耳静脉血中测定了高酮血症诊断时Centrivet和Freestyle的敏感性（Centrivet：50% - 61.3%；Freestyle：93.6% - 75.8%）和特异性（Centrivet GK：75.7% - 73%；Freestyle：37.8% - 70.3%）。Freestyle在颈静脉和耳静脉中低血糖诊断的曲线下面积（AUC）分别为0.89和0.95。Freestyle在颈静脉和耳静脉中低血糖诊断的敏感性分别为60.3%和96.8%。Freestyle在颈静脉和耳静脉中低血糖诊断的特异性分别为100.0%和76.7%。
Freestyle在耳静脉低血糖诊断中表现出可接受的诊断性能，但Freestyle和Centrivet在高酮血症诊断中均未表现出足够的诊断性能。在BHBA和葡萄糖分析中，两种分析仪均不能与RM互换使用。
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