Turgut Ali Osman, Koca Davut, Ünver Ali
Department of Animal Science, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey.
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey.
Reprod Domest Anim. 2024 May;59(5):e14589. doi: 10.1111/rda.14589.
Pregnancy toxaemia is a serious disease that occurs during the last trimester of pregnancy in sheep. Yet, in most cases, the disease may have a subclinical course. This study was aimed at comparing blood βHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in late pregnant sheep. In the study, a total of 50 blood samples were collected from Romanov (n = 30) and cross-bred Hamdani (n = 20) sheep. Blood βHBA levels were measured using TaiDoc TD-4235 and CentriVet βHBA hand-held meter. Randox βHBA (Ranbut) assay was used as a reference laboratory method to compare hand-held meters. βHBA value of 0.8 mmol/L was set as the cut-off value for diagnosis of subclinical pregnancy toxaemia. Statistical analyses were carried out using Minitab 21 and Jamovi software. In the study, the correlation of Randox-TaiDoc TD-4235 and Randox-CentriVet was .822 (p < .001) and .728 (p < .001), respectively. Based on the Ranbut assay, nine ewes out of 50 were diagnosed with subclinical pregnancy toxaemia. Specificity (detection of healthy ewes) and sensitivity (detection of ewes with subclinical pregnancy toxaemia) for TaiDoc TD-4235 and CentriVet hand-held meters were 100%, 77.8%, and 100%, 66.7%, respectively. In the receiver operating characteristic (ROC) analysis, areas under the ROC curve (AUC) were 0.976 and 0.920 for TaiDoc and CentriVet, respectively. Bland-Altman analysis revealed a bias of 0.092 mmol/L for TaiDoc and a bias of 0.132 mmol/L for CentriVet. TaiDoc hand-held meter shows a better correlation with the Randox Ranbut assay and greater sensitivity compared to the CentriVet hand-held meter. In conclusion, both TaiDoc and CentriVet hand-held meters can be securely used in the diagnosis of subclinical pregnancy toxaemia in sheep. For these reasons, subclinical pregnancy toxaemia and these devices will be evaluated within the scope of herd management programme in the sheep industry. It should also be taken into account that these conditions will affect the future fertility of the mother and offspring.
妊娠毒血症是绵羊妊娠晚期发生的一种严重疾病。然而，在大多数情况下，该疾病可能呈亚临床病程。本研究旨在比较用于诊断妊娠晚期绵羊亚临床妊娠毒血症的血液β-羟基丁酸（βHBA）检测设备。在该研究中，共从罗曼诺夫羊（n = 30）和杂交哈姆达尼羊（n = 20）采集了50份血样。使用泰德克TD - 4235和CentriVet βHBA手持式血糖仪测量血液βHBA水平。使用朗道克斯βHBA（Ranbut）检测作为参考实验室方法来比较手持式血糖仪。将βHBA值0.8 mmol/L设定为诊断亚临床妊娠毒血症的临界值。使用Minitab 21和Jamovi软件进行统计分析。在该研究中，朗道克斯 - 泰德克TD - 4235和朗道克斯 - CentriVet的相关性分别为0.822（p < 0.001）和0.728（p < 0.001）。基于Ranbut检测，50只母羊中有9只被诊断为亚临床妊娠毒血症。泰德克TD - 4235和CentriVet手持式血糖仪的特异性（检测健康母羊）和敏感性（检测亚临床妊娠毒血症母羊）分别为100%、77.8%和100%、66.7%。在受试者工作特征（ROC）分析中，泰德克和CentriVet的ROC曲线下面积（AUC）分别为0.976和0.920。布兰德 - 奥特曼分析显示泰德克的偏差为0.092 mmol/L，CentriVet的偏差为0.132 mmol/L。与CentriVet手持式血糖仪相比，泰德克手持式血糖仪与朗道克斯Ranbut检测的相关性更好，敏感性更高。总之，泰德克和CentriVet手持式血糖仪均可安全用于诊断绵羊亚临床妊娠毒血症。基于这些原因，将在绵羊产业的畜群管理计划范围内评估亚临床妊娠毒血症和这些设备。还应考虑到这些情况会影响母羊和后代未来的繁殖能力。
