添加甘草酸（GL）对大黄鱼幼鱼存活、生长性能、摄食相关基因表达、消化酶活性、抗氧化能力及炎症因子表达的影响
Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker () Larvae.
作者信息
Wang Yuntao, Xu Wenxuan, Zhang Jianmin, Liu Jiahui, Wang Zhen, Liu Yongtao, Mai Kangsen, Ai Qinghui
机构信息
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs), Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, China.
出版信息
Aquac Nutr. 2022 Dec 1;2022:5508120. doi: 10.1155/2022/5508120. eCollection 2022.
A 30-day feeding trial was conducted to determine the effects of dietary glycyrrhizin (GL) on survival, growth performance, expression of feeding-related genes, activities of digestive enzymes, antioxidant capacity, and expression of inflammatory factors of large yellow croaker larvae with an initial weight of 3.78 ± 0.27 mg. Four 53.80% crude protein and 16.40% crude lipid diets were formulated with supplementation of 0%, 0.005%, 0.01%, and 0.02% GL, respectively. Results indicated that larvae fed diets with GL had higher survival rate and specific growth rate than the control ( < 0.05). Compared with the control, the mRNA expression of orexigenic factor genes including neuropeptide Y () and agouti-related protein () were significantly increased in larvae fed the diet with 0.005% GL, while the mRNA expression of anorexigenic factor genes including thyrotropin-releasing hormone (), cocaine and amphetamine regulated transcript (), and leptin receptor () were significantly decreased in larvae fed the diet with 0.005% GL ( < 0.05). The trypsin activity in larvae fed the diet with 0.005% GL was significantly higher than the control ( < 0.05). The alkaline phosphatase (AKP) activity in larvae fed the diet with 0.01% GL was significantly higher than the control ( < 0.05). A clear increase of total glutathione (T-GSH) content, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) was observed in larvae fed the diet with 0.01% GL compared with the control ( < 0.05). Moreover, the mRNA expression of interleukin-1 () and interleukin-6 () (proinflammatory genes) in larvae fed the diet with 0.02% GL were significantly lower than the control ( < 0.05). In conclusion, the supplementation of 0.005% -0.01% GL could enhance the expression of orexigenic factor genes, activities of digestive enzymes and antioxidant capacity, ultimately improving the survival, and growth performance of large yellow croaker larvae.
进行了一项为期30天的饲养试验，以确定日粮中甘草甜素（GL）对初始体重为3.78±0.27毫克的大黄鱼幼鱼的存活、生长性能、摄食相关基因表达、消化酶活性、抗氧化能力和炎症因子表达的影响。配制了四种粗蛋白含量为53.80%、粗脂肪含量为16.40%的日粮，分别添加0%、0.005%、0.01%和0.02%的GL。结果表明，投喂含GL日粮的幼鱼存活率和特定生长率高于对照组（P<0.05）。与对照组相比，投喂含0.005%GL日粮的幼鱼中，包括神经肽Y（NPY）和刺鼠相关蛋白（AgRP）在内的促食欲因子基因的mRNA表达显著增加，而投喂含0.005%GL日粮的幼鱼中，包括促甲状腺激素释放激素（TRH）、可卡因和苯丙胺调节转录物（CART）和瘦素受体（LEPR）在内的抑食欲因子基因的mRNA表达显著降低（P<0.05）。投喂含0.005%GL日粮的幼鱼胰蛋白酶活性显著高于对照组（P<0.05）。投喂含0.01%GL日粮的幼鱼碱性磷酸酶（AKP）活性显著高于对照组（P<0.05）。与对照组相比，投喂含0.01%GL日粮的幼鱼总谷胱甘肽（T-GSH）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性明显增加（P<0.05）。此外，投喂含0.02%GL日粮的幼鱼中白细胞介素-1（IL-1）和白细胞介素-6（IL-6）（促炎基因）的mRNA表达显著低于对照组（P<0.05）。总之，添加0.005%-0.01%的GL可以增强促食欲因子基因的表达、消化酶活性和抗氧化能力，最终提高大黄鱼幼鱼的存活率和生长性能。
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