Department of Entomology, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, Minneapolis, MN 55455, USA.
Department of Animal Science, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, Minneapolis, MN 55455, USA.
G3 (Bethesda). 2023 Apr 11;13(4). doi: 10.1093/g3journal/jkad046.
The cecidomyiid fly, soybean gall midge, Resseliella maxima Gagné, is a recently discovered insect that feeds on soybean plants in the Midwestern United States. R. maxima larvae feed on soybean stems that may induce plant death and can cause considerable yield losses, making it an important agricultural pest. From three pools of 50 adults each, we used long-read nanopore sequencing to assemble a R. maxima reference genome. The final genome assembly is 206 Mb with 64.88× coverage, consisting of 1,009 contigs with an N50 size of 714 kb. The assembly is high quality with a Benchmarking Universal Single-Copy Ortholog (BUSCO) score of 87.8%. Genome-wide GC level is 31.60%, and DNA methylation was measured at 1.07%. The R. maxima genome is comprised of 21.73% repetitive DNA, which is in line with other cecidomyiids. Protein prediction annotated 14,798 coding genes with 89.9% protein BUSCO score. Mitogenome analysis indicated that R. maxima assembly is a single circular contig of 15,301 bp and shares highest identity to the mitogenome of the Asian rice gall midge, Orseolia oryzae Wood-Mason. The R. maxima genome has one of the highest completeness levels for a cecidomyiid and will provide a resource for research focused on the biology, genetics, and evolution of cecidomyiids, as well as plant-insect interactions in this important agricultural pest.
叶瘿蚊科的蝇，大豆虫瘿蚊，Resseliella maxima Gagné，是最近在美国中西部发现的一种以大豆植物为食的昆虫。R. maxima 幼虫以大豆茎为食，可能导致植物死亡，并造成相当大的产量损失，因此成为一种重要的农业害虫。我们从三个 50 只成虫的池中，使用长读长纳米孔测序来组装 R. maxima 参考基因组。最终基因组组装大小为 206Mb，覆盖率为 64.88×，由 1009 个 contigs 组成，N50 大小为 714kb。组装质量很高，具有 87.8%的基准通用单拷贝同源物（BUSCO）评分。全基因组 GC 水平为 31.60%，DNA 甲基化水平为 1.07%。R. maxima 基因组由 21.73%的重复 DNA 组成，与其他叶瘿蚊科昆虫一致。蛋白质预测注释了 14798 个编码基因，蛋白质 BUSCO 评分为 89.9%。线粒体基因组分析表明，R. maxima 组装是一个由 15301bp 组成的单一圆形连续序列，与亚洲水稻瘤蚊 Orseolia oryzae Wood-Mason 的线粒体基因组具有最高的同源性。R. maxima 基因组是叶瘿蚊科中完整性水平最高的之一，将为研究叶瘿蚊科的生物学、遗传学和进化，以及这种重要农业害虫的植物-昆虫相互作用提供资源。
