Department of Cellular Biochemistry, University Medical Center, Göttingen, 37073, Germany.
Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, 37075, Germany.
Biol Open. 2023 Mar 15;12(3). doi: 10.1242/bio.059844. Epub 2023 Mar 2.
Mitochondrial defects are associated with aging processes and age-related diseases, including cardiovascular diseases, neurodegenerative diseases and cancer. In addition, some recent studies suggest mild mitochondrial dysfunctions appear to be associated with longer lifespans. In this context, liver tissue is considered to be largely resilient to aging and mitochondrial dysfunction. Yet, in recent years studies report dysregulation of mitochondrial function and nutrient sensing pathways in ageing livers. Therefore, we analyzed the effects of the aging process on mitochondrial gene expression in liver using wildtype C57BL/6N mice. In our analyses, we observed alteration in mitochondrial energy metabolism with age. To assess if defects in mitochondrial gene expression are linked to this decline, we applied a Nanopore sequencing based approach for mitochondrial transcriptomics. Our analyses show that a decrease of the Cox1 transcript correlates with reduced respiratory complex IV activity in older mice livers.
线粒体缺陷与衰老过程和与年龄相关的疾病有关，包括心血管疾病、神经退行性疾病和癌症。此外，一些最近的研究表明，轻微的线粒体功能障碍似乎与更长的寿命有关。在这种情况下，肝脏组织被认为对衰老和线粒体功能障碍有很大的弹性。然而，近年来的研究报告称，衰老肝脏中线粒体功能和营养感应途径的失调。因此，我们使用野生型 C57BL/6N 小鼠分析了衰老过程对肝脏中线粒体基因表达的影响。在我们的分析中，我们观察到随着年龄的增长，线粒体能量代谢发生改变。为了评估线粒体基因表达的缺陷是否与这种下降有关，我们应用了基于纳米孔测序的线粒体转录组学方法。我们的分析表明，Cox1 转录本的减少与老年小鼠肝脏呼吸复合物 IV 活性的降低有关。
