Utlu Bahadır, Öndaş Osman, Yıldırım Mustafa, Bayrakçeken Kemal, Yıldırım Serkan
Department of Ophthalmology, Erzurum Regional Training and Research Hospital, Erzurum, Turkey.
Department of Ophthalmology, Binali Yıldırım University School of Medicine, Erzincan, Turkey.
Eurasian J Med. 2023 Feb;55(1):64-68. doi: 10.5152/eurasianjmed.2023.22234.
The aim of this study is to investigate the effect of topical 5% hesperidin application on healing.
After 48 rats were randomized and divided into 7 groups, on the first day, an epithelial defect was created in the center of the cornea with the help of microkeratome under intraperitoneal ketamine+xylazine and topical 5% proparacaine anesthesia for the groups to be infected with keratitis according to the groups mentioned below. An amount of 0.05 mL of the solution containing 108 colony-forming units/ mL Pseudomonas aeruginosa (PA-ATC27853) will be taken and inoculated per rat. At the end of the 3 days incubation period, rats with keratitis will be added to the groups, and active substances and antibiotics will be given topically together with other groups for 10 days. At the end of the study, the ocular tissues of the rats will be removed and examined histopathologically.
A clinically significant reduction in inflammation was detected in the groups using hesperidin. No transforming growth factor-β1 staining was detected in the group in which keratitis+hesperidin was treated topically. In the group in which hesperidin toxicity was examined, mild inflammation and thickening of the corneal stroma layer were observed, and it was evaluated as a negative transforming growth factor-β1 expression in the lacrimal gland tissue. Corneal epithelial damage was minimal in the keratitis group, and the toxicity group was treated with only hesperidin when compared to the other groups.
Topical hesperidin drops may be an important therapeutic factor in tissue healing and in the fight against inflammation in the treatment of keratitis.
本研究旨在探讨局部应用5%橙皮苷对愈合的影响。
将48只大鼠随机分为7组，第一天，在腹腔注射氯胺酮+赛拉嗪及局部应用5%丙美卡因麻醉下，根据以下分组，用微型角膜刀在角膜中央制作上皮缺损，以便使各组感染角膜炎。每只大鼠取0.05 mL含10⁸菌落形成单位/mL铜绿假单胞菌（PA-ATC27853）的溶液进行接种。在3天的潜伏期结束时，将患有角膜炎的大鼠加入各组，并与其他组一起局部给予活性物质和抗生素，持续10天。研究结束时，取出大鼠的眼组织进行组织病理学检查。
使用橙皮苷的组炎症有显著临床减轻。局部治疗角膜炎+橙皮苷的组未检测到转化生长因子-β1染色。在检查橙皮苷毒性的组中，观察到角膜基质层有轻度炎症和增厚，并评估泪腺组织中转化生长因子-β1表达为阴性。与其他组相比，角膜炎组角膜上皮损伤最小，毒性组仅用橙皮苷治疗。
局部应用橙皮苷滴眼液可能是角膜炎治疗中组织愈合和抗炎的重要治疗因素。
