College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China; Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Int J Biol Macromol. 2022 Aug 1;214:220-229. doi: 10.1016/j.ijbiomac.2022.06.048. Epub 2022 Jun 15.
Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties. In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process. Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively. With the increase of hesperidin ratio, the mean particle size and zeta potential increased gradually. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag was reduced by hesperidin and pectin. Antibacterial tests showed that HP-AgNPs2 showed the MIC values of 66.7 μg/mL against E. coli. In addition, HP-AgNPs2 was selected to clarify its antibacterial mechanism against E. coli. Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS). On the other hand, the release of Ag during cell co-culture was studied and the results showed that most of the Ag released was taken up by E. coli. The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs. These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.
银纳米粒子（AgNPs）由于其优异的抗菌性能而被广泛应用于抗菌领域。在本研究中，我们使用橙皮苷和果胶作为还原剂和稳定剂，通过简单的微波辅助法制备了均匀稳定的橙皮苷-果胶银纳米粒子（HP-AgNPs）。通过增加橙皮苷的比例，分别得到 P-AgNPs、HP-AgNPs1、HP-AgNPs2 和 H-AgNPs。随着橙皮苷比例的增加，平均粒径和zeta 电位逐渐增加。傅里叶变换红外光谱（FTIR）分析表明，Ag 被橙皮苷和果胶还原。抗菌试验表明，HP-AgNPs2 对大肠杆菌的 MIC 值为 66.7μg/mL。此外，选择 HP-AgNPs2 来阐明其对大肠杆菌的抗菌机制。形态学实验表明，HP-AgNPs2 对大肠杆菌细胞壁造成损伤，同时其内容物泄漏并产生更多的活性氧（ROS）。另一方面，研究了细胞共培养过程中 Ag 的释放情况，结果表明，Ag 的大部分释放被大肠杆菌吸收。橙皮苷和果胶的协同作用显著提高了 AgNPs 的抗菌性能。这些初步数据表明，HP-AgNPs 具有良好的抗菌活性，可能被开发为有效的抗菌纳米材料。
