Anesthesiology, Critical Care and Pain Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Pathology Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Sci Rep. 2022 Dec 12;12(1):21465. doi: 10.1038/s41598-022-24639-y.
The cornea is regarded as a sensitive organ to pain. Ketamine can effectively reduce postoperative neuropathic pain. We hypothesized that topical ketamine could mitigate postoperative corneal neuropathic pain. The aim of this study was to determine whether topical ketamine is safe for cornea and evaluate its effect on the repair procedure the damaged corneal tissue. Our study was performed on only the right eyes of 15 male rats. All animals underwent general anesthesia and the whole corneal epithelium was removed. All subjects were divided into two groups: group 1 (n = 8), one drop of ketamine, and group 2 (n = 7), one drop of 0.9% sodium chloride administered topically on the scraped cornea every 6 h for 7 days. The rats' s cornea was carefully monitored daily for the size of epithelial defects under a microscope and was photographed. On the eighth day, the eyes were sent for pathological examination. The eyes were examined for the amount of inflammation, neovascularization, keratinization, epithelial thickness and Descemet's membrane pathologies. The epithelial defect has healed completely on the sixth day in all rats in both groups. There was no significant difference in the speed of complete recovery between the two groups. No significant difference was observed between the two groups in terms of inflammation grade, neovascularization grade, and epithelial thickness. Our study showed that topical ketamine had no significant effect on corneal wound healing in a rat animal model and could be used safely for the management of postoperative chronic ocular pain.
角膜被认为是对疼痛敏感的器官。氯胺酮可以有效减轻术后神经病理性疼痛。我们假设局部使用氯胺酮可以减轻术后角膜神经病理性疼痛。本研究旨在确定局部使用氯胺酮是否安全，以及评估其对受损角膜组织修复过程的影响。我们的研究仅在 15 只雄性大鼠的右眼进行。所有动物均接受全身麻醉，整个角膜上皮被去除。所有动物均分为两组：第 1 组（n=8），给予 1 滴氯胺酮；第 2 组（n=7），给予 1 滴 0.9%氯化钠，每天 6 小时在刮除的角膜上局部给药，持续 7 天。每天在显微镜下仔细监测大鼠角膜上皮缺损的大小，并拍照。第 8 天，将眼睛用于病理检查。检查眼睛的炎症、新生血管形成、角化、上皮厚度和角膜后弹力层病理变化。两组大鼠的角膜上皮缺损均在第 6 天完全愈合。两组之间完全恢复的速度无显著差异。两组之间的炎症分级、新生血管形成分级和上皮厚度无显著差异。我们的研究表明，在大鼠动物模型中，局部使用氯胺酮对角膜伤口愈合没有显著影响，可安全用于治疗术后慢性眼部疼痛。
