Vázquez Loureiro Patricia, Lestido-Cardama Antía, Sendón Raquel, Bustos Juana, Paseiro Losada Perfecto, Rodríguez Bernaldo de Quirós Ana
Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain.
National Food Center, Spanish Agency of Food Safety and Nutrition, Majadahonda, Spain.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2023 Apr;40(4):597-611. doi: 10.1080/19440049.2023.2183051. Epub 2023 Mar 2.
The coatings of metal cans may release complex mixtures of migrants into the contained foods, including non-intentionally added substances (NIAS), such as reaction products. All migrating substances should be studied to demonstrate their safety. In this work, the characterisation of two epoxy and organosol coatings was performed using several techniques. Firstly, the type of coating was identified using FTIR-ATR. Screening techniques based on purge and trap (P&T) and solid-phase microextraction (SPME) coupled to GC-MS were used to investigate volatiles from coatings. For the identification of semi-volatile compounds, an appropriate extraction was performed before analysis by GC-MS. The most abundant substances were compounds with at least one benzene ring and an aldehyde or alcohol group in their structures. Furthermore, a method to quantify some of the identified volatiles was explored. Secondly, HPLC with fluorescence detection (HPLC-FLD) was used to determine non-volatile compounds such as bisphenol analogues and bisphenol A diglycidyl ethers (BADGEs), with subsequent confirmation by LC-MS/MS. Additionally, migration assays were performed by this technique to determine non-volatile compounds migrating into food simulants. Bisphenol A (BPA) and all BADGE derivatives except BADGE.HCl were detected in the migration extracts. Moreover, BADGE-solvent complexes such as BADGE.HO.BuEtOH, BADGE.2BuEtOH, etc. were also tentatively identified using the accurate mass provided by time-of-flight mass spectrometry (TOF-MS).
金属罐的涂层可能会向所盛装的食品中释放复杂的迁移物混合物，包括非有意添加物质（NIAS），如反应产物。应对所有迁移物质进行研究以证明其安全性。在这项工作中，使用了多种技术对两种环氧涂料和有机溶胶涂料进行了表征。首先，使用傅里叶变换红外光谱 - 衰减全反射（FTIR - ATR）来识别涂料类型。基于吹扫捕集（P&T）和固相微萃取（SPME）与气相色谱 - 质谱联用的筛选技术用于研究涂料中的挥发性物质。为了鉴定半挥发性化合物，在气相色谱 - 质谱分析之前进行了适当的萃取。含量最丰富的物质是结构中至少含有一个苯环以及一个醛基或醇基的化合物。此外，还探索了一种定量某些已鉴定挥发性物质的方法。其次，使用带荧光检测的高效液相色谱（HPLC - FLD）来测定双酚类似物和双酚A二缩水甘油醚（BADGEs）等非挥发性化合物，随后通过液相色谱 - 串联质谱（LC - MS/MS）进行确证。此外，通过该技术进行迁移试验以测定迁移到食品模拟物中的非挥发性化合物。在迁移提取物中检测到了双酚A（BPA）和除BADGE.HCl之外的所有BADGE衍生物。此外，还利用飞行时间质谱（TOF - MS）提供的精确质量初步鉴定了BADGE - 溶剂配合物，如BADGE.HO.BuEtOH、BADGE.2BuEtOH等。
