Xiao Chi, Ross Georgina, Nielen Michel W F, Eriksson Jens, Salentijn Gert Ij, Mak Wing Cheung
Division of Sensor and Actuator Systems, IFM - Linköping University, S58183, Linköping, Sweden.
Wageningen Food Safety Research (WFSR), Wageningen University & Research, P.O. Box 230, 6700, AE, Wageningen, the Netherlands; Laboratory of Organic Chemistry, Wageningen University, Helix Building 124, Stippeneng 4, 6708 WE, Wageningen, the Netherlands.
Talanta. 2023 May 15;257:124366. doi: 10.1016/j.talanta.2023.124366. Epub 2023 Feb 20.
Food allergies are hypersensitivity immune responses triggered by (traces of) allergenic compounds in foods and drinks. The recent trend towards plant-based and lactose-free diets has driven an increased consumption of plant-based milks (PBMs) with the risk of cross-contamination of various allergenic plant-based proteins during the food manufacturing process. Conventional allergen screening is usually performed in the laboratory, but portable biosensors for on-site screening of food allergens at the production site could improve quality control and food safety. Here, we developed a portable smartphone imaging surface plasmon resonance (iSPR) biosensor composed of a 3D-printed microfluidic SPR chip for the detection of total hazelnut protein (THP) in commercial PBMs and compared its instrumentation and analytical performance with a conventional benchtop SPR. The smartphone iSPR shows similar characteristic sensorgrams compared with the benchtop SPR and enables the detection of trace levels of THP in spiked PBMs with the lowest tested concentration of 0.625 μg/mL THP. The smartphone iSPR achieved LoDs of 0.53, 0.16, 0.14, 0.06, and 0.04 μg/mL THP in 10x-diluted soy, oat, rice, coconut, and almond PBMs, respectively, with good correlation with the conventional benchtop SPR system (R 0.950-0.991). The portability and miniaturized characteristics of the smartphone iSPR biosensor platform make it promising for the future on-site detection of food allergens by food producers.
食物过敏是由食品和饮料中的（微量）致敏化合物引发的超敏免疫反应。最近向植物性和无乳糖饮食的趋势促使植物性奶（PBMs）的消费量增加，在食品制造过程中存在各种致敏植物性蛋白质交叉污染的风险。传统的过敏原筛查通常在实验室进行，但用于在生产现场对食物过敏原进行现场筛查的便携式生物传感器可以改善质量控制和食品安全。在此，我们开发了一种便携式智能手机成像表面等离子体共振（iSPR）生物传感器，它由一个3D打印的微流控SPR芯片组成，用于检测市售PBMs中的总榛子蛋白（THP），并将其仪器和分析性能与传统台式SPR进行了比较。与台式SPR相比，智能手机iSPR显示出相似的特征传感图，并能够检测加标PBMs中痕量水平的THP，最低测试浓度为0.625μg/mL THP。智能手机iSPR在10倍稀释的大豆、燕麦、大米、椰子和杏仁PBMs中分别实现了0.53、0.16、0.14、0.06和0.04μg/mL THP的检测限，与传统台式SPR系统具有良好的相关性（R 0.950 - 0.991）。智能手机iSPR生物传感器平台的便携性和小型化特性使其有望在未来被食品生产商用于食物过敏原的现场检测。
