Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy.
Department of Medicine, University of Udine, Udine, Italy.
Pest Manag Sci. 2023 Jul;79(7):2503-2516. doi: 10.1002/ps.7435. Epub 2023 Mar 16.
In recent years, biofungicides have drawn increasing interest in vineyards for a more sustainable integrated and copper-limited pest management. Among alternatives, botanicals could represent valuable tools, being rich sources of biologically active compounds. Conversely to the well-known antioxidant and biological properties in relation to health benefits, investigation on bioactivity of hot pungent Capsicum sp. products against fungal phytopathogens in vineyards is still scarce. Therefore, the present study aimed at exploring the biologically active compounds profile of a chili pepper (Capsicum chinense Jacq.) pod extract and its antimicrobial properties against some of the major fungal and Oomycetes pathogens of grapevine, including Botrytis cinerea Pers., Guignardia bidwellii (Ellis) Viala & Ravaz and Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni.
The ethyl acetate-extracted oleoresin from the most pungent varieties was rich in capsaicinoids and polyphenols (371.09 and 268.5 μg mg dry weight, respectively). Capsaicin and dihydrocapsaicin, hydroxycinnamic and hydroxybenzoic acids and quercetin derivatives were the most abundant, while carotenoids represented only a minor fraction. The oleoresin was efficient to inhibit all three pathogenic fungi and ED values were determined, evidencing that G. bidwellii was the more sensitive (0.233 ± 0.034 mg mL ).
The results suggested a potentiality of chili pepper extract for the control of some important grapevine pathogens, their possible application being helpful for the recommended limitation in extensive use of copper in vineyard. The complex mixture of high amounts of capsaicinoids, associated to specific phenolic acids and other minor bioactive components might contribute to the observed antimicrobial action of chili pepper extract. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
近年来，生物杀菌剂在葡萄园中的应用越来越受到关注，以实现更可持续的综合和铜限量害虫管理。在替代方法中，植物提取物可能是有价值的工具，因为它们是生物活性化合物的丰富来源。与抗氧化和生物特性相关的众所周知的健康益处相反，关于辣椒（Capsicum chinense Jacq.）豆荚提取物对葡萄园真菌病原菌的生物活性的研究仍然很少。因此，本研究旨在探索一种辣椒（Capsicum chinense Jacq.）豆荚提取物的生物活性化合物谱及其对一些主要真菌和卵菌病原菌的抗菌特性，包括灰葡萄孢（Botrytis cinerea Pers.）、钩丝壳菌（Guignardia bidwellii （Ellis） Viala & Ravaz）和葡萄生单轴霉（Plasmopara viticola （Berk. & M.A. Curtis） Berl. & De Toni）。
最辣品种的乙酸乙酯提取物富含辣椒素类物质和多酚（分别为 371.09 和 268.5μg·mg 干重）。辣椒素和二氢辣椒素、羟基肉桂酸和羟基苯甲酸以及槲皮素衍生物是最丰富的，而类胡萝卜素只占一小部分。该油树脂能有效抑制三种致病真菌，测定 ED 值，表明钩丝壳菌最敏感（0.233±0.034mg·mL-1）。
研究结果表明，辣椒提取物具有控制一些重要葡萄病原菌的潜力，其可能的应用有助于推荐限制在葡萄园中广泛使用铜。高含量的辣椒素类物质与特定的酚酸和其他少量生物活性成分的复杂混合物可能有助于观察到辣椒提取物的抗菌作用。© 2023 作者。《农药科学》由 John Wiley & Sons Ltd 代表化学工业协会出版。
