Kulisch Ágota, Mándó Zsuzsanna, Sándor Enikő, Lengyel Zsuzsanna, Illés Anett, Kósa János, Árvai Kristóf, Lakatos Péter, Tóbiás Bálint, Papp Márton, Bender Tamás
St. Andrew Hospital for Rheumatic Diseases, Schulhof Vilmos sétány 1., Hévíz, 8380, Hungary.
Department of Dermatology, Venerology and Oncodermatology, Medical School, Clinical Center, University of Pécs, Akác utca 1., Pécs, 7632, Hungary.
Int J Biometeorol. 2023 Apr;67(4):661-673. doi: 10.1007/s00484-023-02443-1. Epub 2023 Mar 3.
Psoriasis is a chronic inflammatory skin disease. It is associated with changes in skin microbiome. The aim of this study was to evaluate how Lake Hévíz sulfur thermal water influences the composition of microbial communities that colonizes skin in patients with psoriasis. Our secondary objective was to investigate the effects of balneotherapy on disease activity. In this open label study, participants with plaque psoriasis underwent 30-min therapy sessions in Lake Hévíz, at a temperature of 36 °C, five times a week for 3 weeks. The skin microbiome samples were collected by swabbing method from two different areas (lesional skin-psoriatic plaque and non-lesional skin). From 16 patients, 64 samples were processed for a 16S rRNA sequence-based microbiome analysis. Outcome measures were alpha-diversity (Shannon, Simpson, and Chao1 indexes), beta-diversity (Bray-Curtis metric), differences in genus level abundances, and Psoriasis Area and Severity Index (PASI). Skin microbiome samples were collected at baseline, and immediately after treatment. Based on the visual examination of the employed alpha- and beta-diversity measures, no systematic difference based on sampling timepoint or sample location could be revealed in these regards. Balneotherapy in the unaffected area significantly increased the level of Leptolyngbya genus, and significantly decreased the level of Flavobacterium genus. A similar trend was revealed by the results of the psoriasis samples, but the differences were not statistically significant. In patients with mild psoriasis, a significant improvement was observed in PASI scores.
银屑病是一种慢性炎症性皮肤病。它与皮肤微生物群的变化有关。本研究的目的是评估赫维兹湖硫磺温泉水如何影响银屑病患者皮肤定植的微生物群落组成。我们的次要目标是研究温泉疗法对疾病活动的影响。在这项开放标签研究中，斑块状银屑病患者在赫维兹湖接受30分钟的治疗，水温为36°C，每周5次，共3周。通过擦拭法从两个不同区域（皮损皮肤——银屑病斑块和非皮损皮肤）采集皮肤微生物群样本。对16名患者的64个样本进行基于16S rRNA序列的微生物群分析。观察指标为α多样性（香农指数、辛普森指数和Chao1指数）、β多样性（布雷-柯蒂斯度量）、属水平丰度差异以及银屑病面积和严重程度指数（PASI）。在基线时以及治疗后立即采集皮肤微生物群样本。基于对所采用的α多样性和β多样性测量指标的视觉检查，在这些方面未发现基于采样时间点或样本位置的系统差异。非患病区域的温泉疗法显著增加了鞘丝藻属的水平，并显著降低了黄杆菌属的水平。银屑病样本的结果也显示出类似趋势，但差异无统计学意义。在轻度银屑病患者中，观察到PASI评分有显著改善。
