Nataliia Raksha, Volodymyrska Street 64/13, 01601 Kyiv, Ukraine,
Croat Med J. 2023 Feb 28;64(1):21-28. doi: 10.3325/cmj.2023.64.21.
To investigate the ability of collagen peptides derived from a jellyfish of the Antarctic region (Diplulmaris antarctica) to prevent the development of obesity in rats fed a high-calorie diet.
Collagen peptides were produced by pepsin hydrolysis of jellyfish-derived collagen. The purity of collagen and collagen peptides was confirmed by SDS-polyacrylamide gel electrophoresis. Rats were fed a high-calorie diet for ten weeks and were simultaneously orally administered collagen peptides (1 g per 1 kg of body weight every other day) starting from the fourth week. Body mass index (BMI), body weight gain, selected nutritional parameters, the key parameters associated with insulin resistance, and the level of oxidative stress markers were assessed.
Compared with untreated obese rats, rats treated with hydrolyzed jellyfish collagen peptides had a decreased body weight gain and body mass index. They also had a decreased level of fasting blood glucose, glycated hemoglobin, insulin, lipid peroxidation products (conjugated dienes, Schiff bases), and oxidatively modified proteins, as well as a restored activity of superoxide dismutase.
Collagen peptides obtained from Diplulmaris antarctica can be used to prevent and treat obesity caused by a high-calorie diet and pathologies associated with increased oxidative stress. Given the obtained results and the abundance of Diplulmaris antarctica in the Antarctic region, this species can be considered a sustainable source of collagen and its derivatives.
研究源自南极地区水母（Diplulmaris antarctica）的胶原蛋白肽预防高热量饮食喂养大鼠肥胖发展的能力。
通过胃蛋白酶水解水母衍生胶原蛋白生产胶原蛋白肽。通过 SDS-聚丙烯酰胺凝胶电泳确认胶原蛋白和胶原蛋白肽的纯度。大鼠喂养高热量饮食十周，并从第四周开始每天口服胶原蛋白肽（每 1 公斤体重 1 克）。评估体重指数（BMI）、体重增加、选定的营养参数、与胰岛素抵抗相关的关键参数以及氧化应激标志物的水平。
与未处理的肥胖大鼠相比，用水解水母胶原蛋白肽处理的大鼠体重增加和体重指数降低。它们的空腹血糖、糖化血红蛋白、胰岛素、脂质过氧化产物（共轭二烯、席夫碱）和氧化修饰蛋白水平也降低，超氧化物歧化酶活性恢复。
源自 Diplulmaris antarctica 的胶原蛋白肽可用于预防和治疗高热量饮食引起的肥胖以及与氧化应激增加相关的病理学。鉴于获得的结果和南极地区 Diplulmaris antarctica 的丰富度，该物种可以被认为是胶原蛋白及其衍生物的可持续来源。
