在细胞质中积累分泌蛋白前体的大肠杆菌突变体。

Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm.

作者信息

Bassford P, Beckwith J

出版信息

Nature. 1979 Feb 15;277(5697):538-41. doi: 10.1038/277538a0.

Abstract

The maltose binding protein of Escherichia coli is secreted into the external periplasmic compartment of the cell. This article describes a selection procedure for the isolation of mutants which fail to export this protein. These mutants probably result from alterations in the amino terminal 'signal sequence', causing the maltose binding protein produced to accumulate in the cytoplasm in its precursor form.

摘要

大肠杆菌的麦芽糖结合蛋白被分泌到细胞的外周质区室中。本文描述了一种用于分离不能输出该蛋白的突变体的筛选程序。这些突变体可能是由于氨基末端“信号序列”的改变所致，使得所产生的麦芽糖结合蛋白以前体形式积累在细胞质中。