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大肠杆菌麦芽糖结合蛋白（MBP）的成熟部分决定了MBP对SecB的输出依赖性。

The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export.

作者信息

Gannon P M, Li P, Kumamoto C A

机构信息

Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111.

出版信息

J Bacteriol. 1989 Feb;171(2):813-8. doi: 10.1128/jb.171.2.813-818.1989.

DOI:10.1128/jb.171.2.813-818.1989
PMID:2644237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC209669/
Abstract

The product of the secB gene is required for export of a subset of secreted proteins to the outer membrane and periplasm of Escherichia coli. Precursor maltose-binding protein (MBP) accumulates in the cytoplasm of secB-carrying mutants, but export of alkaline phosphatase is only minimally affected by secB mutations. When export of MBP-alkaline phosphatase hybrid proteins was analyzed in wild-type and secB-carrying mutant strains, the first third of mature MBP was sufficient to render export of the hybrid proteins dependent on SecB. Substitution of a signal sequence from a SecB-independent protein had no effect on SecB-dependent export. These findings show that the first third of mature MBP is capable of conferring export incompetence on an otherwise competent protein.

摘要

secB基因的产物是大肠杆菌中一部分分泌蛋白输出至外膜和周质所必需的。前体麦芽糖结合蛋白（MBP）在携带secB的突变体细胞质中积累，但碱性磷酸酶的输出仅受到secB突变的轻微影响。当在野生型和携带secB的突变体菌株中分析MBP-碱性磷酸酶杂合蛋白的输出时，成熟MBP的前三分之一足以使杂合蛋白的输出依赖于SecB。用来自不依赖SecB的蛋白的信号序列进行替换，对依赖SecB的输出没有影响。这些发现表明，成熟MBP的前三分之一能够使原本可输出的蛋白变得无法输出。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/fa4ccdc74347/jbacter00168-0206-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/8ca5f5035e29/jbacter00168-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/9745962e7346/jbacter00168-0205-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/c084ff4fc9ab/jbacter00168-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/bdb57aee298a/jbacter00168-0206-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/fa4ccdc74347/jbacter00168-0206-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/8ca5f5035e29/jbacter00168-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/9745962e7346/jbacter00168-0205-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/c084ff4fc9ab/jbacter00168-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/bdb57aee298a/jbacter00168-0206-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d67/209669/fa4ccdc74347/jbacter00168-0206-c.jpg

相似文献

1
The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export.大肠杆菌麦芽糖结合蛋白（MBP）的成熟部分决定了MBP对SecB的输出依赖性。
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2
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3
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4
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SecB-independent export of Escherichia coli ribose-binding protein (RBP): some comparisons with export of maltose-binding protein (MBP) and studies with RBP-MBP hybrid proteins.大肠杆菌核糖结合蛋白（RBP）不依赖SecB的输出：与麦芽糖结合蛋白（MBP）输出的一些比较以及对RBP-MBP杂合蛋白的研究。
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本文引用的文献

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E. coli mutant pleiotropically defective in the export of secreted proteins.大肠杆菌突变体在分泌蛋白输出方面存在多效性缺陷。
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