Nguyen Huong Thi Mai, Lee Der-Yen, Liu Chung-Hsiang, Hsieh Ching-Liang
Graduate Institute of Acupuncture Science, China Medical University, Taichung, Taiwan.
Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan.
Evid Based Complement Alternat Med. 2023 Feb 21;2023:4849528. doi: 10.1155/2023/4849528. eCollection 2023.
This study investigated changes in neurotransmitters induced by the application of electroacupuncture (EA) at Zusanli (ST36) and Neiguan (PC6). A total of 30 rats were divided into five groups: sham, ST (EA at bilateral ST36 and ST37), ScT (ST plus previous neurectomy of the bilateral sciatic nerves), ScS (sham plus previous neurectomy of the bilateral sciatic nerve), and PC (EA at bilateral PC6 and PC7). The P2X2 receptor expression was stronger in the sham group than in the ST and PC groups (both < 0.05) but similar between the sham and ScT groups ( > 0.05). Dopamine levels in the extracellular fluid surrounding the acupoints were higher in the PC group than in the sham and ST groups during the postacupuncture period (both < 0.05). Glutamate levels in the extracellular fluid surrounding the acupoints were higher in the ST group than in the sham group during the acupuncture period ( < 0.05) and higher in the ST group than in the sham and PC groups during the postacupuncture period (both < 0.05). Serum adrenaline and noradrenaline levels were higher in the PC group than in the sham, ST, and ScT groups (all < 0.05). Glutamate levels in the CSF were higher in the ST group than in the sham, ScS, and PC groups (all < 0.05). GABA levels in the CSF were higher in the ST group than in the sham, ScT, and PC groups (all < 0.05). EA at ST36 and ST37 and PC6 and PC7 exerted an analgesic effect, EA at PC6 and PC7 can enhance heart function, and EA at ST36 and ST37 modulates the cerebral cortex. However, the study needs an evaluation of direct pain behavior, heart function, and brain function in the future.
本研究调查了针刺足三里（ST36）和内关（PC6）所诱导的神经递质变化。总共30只大鼠被分为五组：假手术组、ST组（双侧ST36和ST37电针）、ScT组（ST组加双侧坐骨神经预先切除）、ScS组（假手术组加双侧坐骨神经预先切除）和PC组（双侧PC6和PC7电针）。假手术组的P2X2受体表达强于ST组和PC组（均P<0.05），但假手术组和ScT组之间相似（P>0.05）。针刺后阶段，PC组穴位周围细胞外液中的多巴胺水平高于假手术组和ST组（均P<0.05）。针刺期间，ST组穴位周围细胞外液中的谷氨酸水平高于假手术组（P<0.05），针刺后阶段，ST组的谷氨酸水平高于假手术组和PC组（均P<0.05）。PC组血清肾上腺素和去甲肾上腺素水平高于假手术组、ST组和ScT组（均P<0.05）。ST组脑脊液中的谷氨酸水平高于假手术组、ScS组和PC组（均P<0.05）。ST组脑脊液中的γ-氨基丁酸水平高于假手术组、ScT组和PC组（均P<0.05）。ST36和ST37以及PC6和PC7电针具有镇痛作用，PC6和PC7电针可增强心脏功能，ST36和ST37电针可调节大脑皮层。然而，本研究未来需要对直接疼痛行为、心脏功能和脑功能进行评估。
