Qi Xiao, Li Zihao, Zhang Jinrui, Li Hongru, Zhang Guangxin, Li Meng, Li Baofeng, Fu Yilin, Cai Mingjun, Wang Hongda, Tong Ti, Gao Jing
Department of Thoracic Surgery, the Second Hospital of Jilin University, Changchun, Jilin 130041, China.
State Key Laboratory of Electroanalytical Chemistry, Research Center of Biomembranomics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
iScience. 2023 Feb 2;26(3):106103. doi: 10.1016/j.isci.2023.106103. eCollection 2023 Mar 17.
CDCP1 is a transmembrane protein that is involved in a variety of important biological processes and upregulated in a variety of human solid malignancies; however, its spatial distribution and variation at the molecular level remain unclear. To solve this problem, we first analyzed its expression level and prognostic implications in lung cancer. Then, we used super-resolution microscopy to reveal the spatial organization of CDCP1 at different levels, and found that cancer cells generated more and larger CDCP1 clusters than normal cells. Furthermore, we found that CDCP1 can be integrated into larger and denser clusters as functional domains upon activation. Our findings elucidated the significant differences of CDCP1 clustering characteristics between cancer and normal cells, and revealed the relationship between its distribution and function, which will contribute to a comprehensive understanding of its oncogenic mechanism, and will be of great help for the development of CDCP1-targeted drugs for lung cancer.
CDCP1是一种跨膜蛋白，参与多种重要的生物学过程，且在多种人类实体恶性肿瘤中上调；然而，其在分子水平上的空间分布和变异仍不清楚。为了解决这个问题，我们首先分析了它在肺癌中的表达水平和预后意义。然后，我们使用超分辨率显微镜揭示了不同水平下CDCP1的空间组织，发现癌细胞比正常细胞产生更多、更大的CDCP1簇。此外，我们发现CDCP1在激活后可作为功能域整合到更大、更密集的簇中。我们的研究结果阐明了癌症细胞与正常细胞之间CDCP1簇集特征的显著差异，并揭示了其分布与功能之间的关系，这将有助于全面了解其致癌机制，对开发针对肺癌的CDCP1靶向药物有很大帮助。
