ICAR, Central Institute of Fisheries Education, Mumbai, India.
TNJFU-Institute of Fisheries Post Graduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, Tamil Nadu, India.
Anim Biotechnol. 2023 Dec;34(8):3609-3616. doi: 10.1080/10495398.2023.2184697. Epub 2023 Mar 3.
A 60-day feeding trial was conducted to study the hematology, liver, and intestinal histoarchitecture of fed with a combination of exogenous enzymes, essential amino acids, and essential fatty acids to DORB (De-oiled rice bran) based diets. Three treatments viz., T1 [DORB + phytase and xylanase (0.01% each)], T2 [DORB + phytase (0.01%) + xylanase (0.01%) + L-lysine(1.4%) + L-methionine (0.4%) + EPA and DHA (0.5%)] and T3 [DORB + phytase (0.01%), xylanase and cellulase (0.075%) + L-lysine (1.4%) +L-methionine (0.4%) + EPA and DHA (0.5%)] were used in the present study. Serum total protein, albumin content and A/G ratio varied significantly ( < 0.05) among groups. Globulin content did not vary significantly among groups ( ≥ 0.05). The Hb content, RBC and MCV count varied significantly ( < 0.05) whereas MCH, MCHC content, WBC and lymphocyte count did not vary significantly among groups ( > 0.05). The liver and intestine examination revealed no visible alteration and showed normal histo-architecture. Based on the finding it is concluded that DORB supplemented with exogenous enzymes, essential amino acids and essential fatty acids with phytase (0.01%), xylanase and cellulase (0.075%), L-lysine (1.4%), DL-methionine (0.4%) and EPA and DHA (0.5%) improves the health of .
进行了为期 60 天的饲养试验,以研究用外源性酶、必需氨基酸和必需脂肪酸组合喂养的猪的血液学、肝脏和肠道组织学结构,这些饲料基于脱油米糠(DORB)。本研究使用了三种处理方法:T1 [DORB+植酸酶和木聚糖酶(各 0.01%)]、T2 [DORB+植酸酶(0.01%)+木聚糖酶(0.01%)+L-赖氨酸(1.4%)+L-蛋氨酸(0.4%)+EPA 和 DHA(0.5%)] 和 T3 [DORB+植酸酶(0.01%)、木聚糖酶和纤维素酶(0.075%)+L-赖氨酸(1.4%)+L-蛋氨酸(0.4%)+EPA 和 DHA(0.5%)]。血清总蛋白、白蛋白含量和 A/G 比值在各组间差异显著( < 0.05)。球蛋白含量在各组间无显著差异( ≥ 0.05)。血红蛋白含量、红细胞和 MCV 计数在各组间差异显著( < 0.05),而 MCH、MCHC 含量、白细胞和淋巴细胞计数在各组间无显著差异( > 0.05)。肝脏和肠道检查未发现明显变化,组织学结构正常。基于这些发现,可以得出结论,用外源性酶、必需氨基酸和必需脂肪酸补充脱油米糠,并添加植酸酶(0.01%)、木聚糖酶和纤维素酶(0.075%)、L-赖氨酸(1.4%)、DL-蛋氨酸(0.4%)和 EPA 和 DHA(0.5%)可以改善猪的健康状况。