[甘油和聚乙二醇对质膜的冷冻保护作用的有效性]
[Effectiveness of the cryoprotective effect of glycerol and polyethylene glycol on plasma membranes].
作者信息
Riazantsev V V, Gulevskiĭ A K
出版信息
Ukr Biokhim Zh (1978). 1987 Sep-Oct;59(5):97-9.
The barrier properties of erythrocyte membranes were studied under freezing-thawing in cryopreservatives based on glycerol or polyethylene glycol Mm 1500 Da (PEG-1500) (at cryoprotectant concentrations from 0.5 to 30%). The state of the membrane barrier function in reconstituted and native erythrocytes subjected to rapid (200-400 degrees C per min) freezing has been registered by spectrophotometry, flame photometry, and radiolabel analysis as judged by labeled compound release (hemoglobin, K+, [14C]sucrose, 86Rb+). It is demonstrated that the effective cryoprotection of erythrocyte membranes in reconstituted erythrocytes is attained at cryoprotectant concentrations in the medium of 0.5-1% or in erythrocytes of 8-30%.
在基于甘油或聚乙二醇Mm 1500 Da（PEG - 1500）的冷冻保护剂中进行冻融处理时，研究了红细胞膜的屏障特性（冷冻保护剂浓度为0.5%至30%）。通过分光光度法、火焰光度法和放射性标记分析，根据标记化合物释放（血红蛋白、K⁺、[¹⁴C]蔗糖、⁸⁶Rb⁺）来记录快速（每分钟200 - 400摄氏度）冷冻的重构红细胞和天然红细胞中膜屏障功能的状态。结果表明，在重构红细胞中，当培养基中冷冻保护剂浓度为0.5 - 1%或红细胞中为8 - 30%时，可实现对红细胞膜的有效冷冻保护。
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