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[跨膜电位在冷冻保存过程中损害红细胞膜屏障特性中的作用]

[Role of the transmembrane potential in impairing the barrier properties of erythrocyte membranes during cryopreservation].

作者信息

Gulevskiĭ A K, Riazantsev V V, Kukushkin A I

出版信息

Biull Eksp Biol Med. 1985 Dec;100(12):690-1.

PMID:4074868
Abstract

Exposure of erythrocytes to nonpermeable cryoprotector solution (PEO-1500, sucrose) with reduced NaCl content resulted in the development of chlorine diffusion potential, calculated by the degree of medium acidulation. This was accompanied by the loss of intracellular K+ and the increase in hemolysis upon freezing and thawing.

摘要

将红细胞暴露于NaCl含量降低的非渗透性冷冻保护剂溶液(PEO - 1500、蔗糖)中,会导致氯扩散电位的产生,这是通过培养基酸化程度计算得出的。这伴随着细胞内K⁺的流失以及冻融时溶血的增加。

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Biull Eksp Biol Med. 1985 Dec;100(12):690-1.
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