Gordiyenko O I, Anikieieva M O, Rozanova S L, Kovalenko S Ye, Kovalenkol I F, Gordiyenko E O
Cryo Letters. 2015 May-Jun;36(3):221-6.
Maintaining cell surface properties after freezing and thawing, characterized in particular by the surface potential and associated with it cell ability to intercellular adhesion, could be used as a characteristic of successful cryopreservation.
This study was conducted to research applying different erythrocytes freezing modes and analyses the regimes cryopreservation effect on the cell surface charge and adhesion to microorganisms.
Human erythrocytes frozen by three modes. In order to determine adhesion index was used dried bacterial cells of S. thermophilus. The surface charge of erythrocytes was evaluated using Alcian blue cationic dye.
The results showed the significant decrease in the lactobacillus adhesion to erythrocytes frozen glycerol and 1,2-propanediol. After erythrocytes were freezen with glycerol and 1,2-propanediol, the cationic dye binding to erythrocytes significantly reduced. AB binding to erythrocytes frozen with PEG-1500 does not differ from control data.
Erythrocytes frozen with PEG-1500 mantained surface properties after thawing better, compared to erythrocytes cryopreserved by other methods.
冻融后维持细胞表面特性，特别是以表面电位为特征并与之相关的细胞间黏附能力，可作为成功冷冻保存的一个特征。
本研究旨在探讨应用不同的红细胞冷冻模式，并分析冷冻保存方案对细胞表面电荷及对微生物黏附的影响。
采用三种模式冷冻人红细胞。为测定黏附指数，使用嗜热链球菌的干燥细菌细胞。使用阿尔新蓝阳离子染料评估红细胞的表面电荷。
结果显示，乳酸杆菌对用甘油和1,2 - 丙二醇冷冻的红细胞的黏附显著降低。在用甘油和1,2 - 丙二醇冷冻红细胞后，阳离子染料与红细胞的结合显著减少。用聚乙二醇 - 1500（PEG - 1500）冷冻的红细胞与阿尔新蓝的结合与对照数据无差异。
与其他方法冷冻保存的红细胞相比，用聚乙二醇 - 1500冷冻的红细胞解冻后能更好地维持表面特性。
