Pharmacy College of, Changchun University of Chinese Medicine, Changchun, 130117, China.
Pharmacy College of, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Chem Biodivers. 2023 Apr;20(4):e202200949. doi: 10.1002/cbdv.202200949. Epub 2023 Mar 20.
This study investigated the effect of butanol extract of AS (ASBUE) on atherosclerosis in apolipoprotein E-deficient (ApoE mice. The mice were administered ASBUE (390 or 130 mg/kg/day) or rosuvastatin (RSV) via oral gavage for eight weeks. In ApoE mice, ASBUE suppressed the abnormal body weight gain and improved serum and liver biochemical indicators. ASBUE remarkably reduced the aortic plaque area, improved liver pathological conditions, and lipid metabolism abnormalities, and altered the intestinal microbiota structure in ApoE mice. In the vascular tissue of ASBUE-treated mice, P-IKKβ, P-NFκB, and P-IκBα levels tended to decrease, while IκB-α increased in high fat-diet-fed atherosclerotic mice. These findings demonstrated the anti-atherosclerotic potential of ASBUE, which is mediated by the interaction between the gut microbiota and lipid metabolism and regulated via the Nuclear Factor-kappa B (NF-κB) pathway. This work paves the groundwork for subsequent studies to develop innovative drugs to treat atherosclerosis.
本研究探讨了 AS 的丁醇提取物（ASBUE）对载脂蛋白 E 缺陷（ApoE ）小鼠动脉粥样硬化的影响。将 ASBUE（390 或 130mg/kg/天）或瑞舒伐他汀（RSV）通过口服灌胃给予 ApoE 小鼠 8 周。在 ApoE 小鼠中，ASBUE 抑制了异常的体重增加并改善了血清和肝脏生化指标。ASBUE 显著减少了主动脉斑块面积，改善了肝脏病理状况和脂质代谢异常，并改变了 ApoE 小鼠的肠道微生物群结构。在接受 ASBUE 治疗的小鼠的血管组织中，高脂饮食喂养的动脉粥样硬化小鼠中 P-IKKβ、P-NFκB 和 P-IκBα 水平趋于降低，而 IκB-α 增加。这些发现表明 ASBUE 具有抗动脉粥样硬化的潜力，这是由肠道微生物群和脂质代谢的相互作用介导的，并通过核因子-kappa B（NF-κB）途径进行调节。这项工作为后续研究开发治疗动脉粥样硬化的创新药物奠定了基础。
