Pardeshi Priya, Jadhav Pravin, Sakhare Sanjay, Zunjare Rajkumar, Rathod Darasing, Sonkamble Priti, Saroj Ranjit, Varghese Philips
Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India.
ICAR-Indian Agricultural Research Institute, New Delhi, India.
Mol Biol Rep. 2023 May;50(5):4049-4060. doi: 10.1007/s11033-023-08328-1. Epub 2023 Mar 4.
Vegetable soybean seeds are among the most popular and nutrient-dense beans in the world due to their delicious flavor, high yield, superior nutritional value, and low trypsin content. There is significant potential for this crop that Indian farmers do not fully appreciate because of the limited germplasm range. Therefore, the current study aims to identify the diverse lines of vegetable soybean and explore the diversity produced by hybridizing grain and vegetable-type soybean varieties. Indian researchers have not yet published work describing and analysing novel vegetable soybean for microsatellite markers and morphological traits.
Sixty polymorphic SSR markers and 19 morphological traits were used to evaluate the genetic diversity of 21 newly developed vegetable soybean genotypes. A total of 238 alleles, ranging from 2 to 8, were found, with a mean of 3.97 alleles per locus. The polymorphism information content varied from 0.05 to 0.85, with an average of 0.60. A variation of 0.25-0.58 with a mean of 0.43 was observed for Jaccard's dissimilarity coefficient.
The diverse genotypes identified can be helpful to understand the genetics of vegetable soybean traits and can be used in improvement programs; study also explains the utility of SSR markers for diversity analysis of vegetable soybean. Here, we identified the highly informative SSRs with PIC > 0.80 (satt199, satt165, satt167, satt191, satt183, satt202, and satt126), which apply to genetic structure analysis, mapping strategies, polymorphic marker surveys, and background selection in genomics-assisted breeding.
毛豆种子因其美味的口感、高产、卓越的营养价值和低胰蛋白酶含量，成为世界上最受欢迎且营养丰富的豆类之一。由于种质范围有限，印度农民尚未充分认识到这种作物的巨大潜力。因此，本研究旨在鉴定毛豆的不同品系，并探索通过杂交谷物型和蔬菜型大豆品种产生的多样性。印度研究人员尚未发表关于描述和分析新型毛豆微卫星标记及形态特征的研究成果。
使用60个多态性SSR标记和19个形态特征来评估21个新培育的毛豆基因型的遗传多样性。共发现238个等位基因，范围从2到8个，平均每个位点3.97个等位基因。多态性信息含量在0.05至0.85之间，平均为0.60。Jaccard's差异系数的变化范围为0.25 - 0.58，平均值为0.43。
鉴定出的不同基因型有助于理解毛豆性状的遗传学，并可用于改良计划；该研究还解释了SSR标记在毛豆多样性分析中的实用性。在此，我们鉴定出了PIC > 0.80的高信息量SSR（satt199、satt165、satt167、satt191、satt183、satt202和satt126），这些标记适用于基因组辅助育种中的遗传结构分析、作图策略、多态性标记调查和背景选择。
