State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Food Chem. 2023 Jul 30;415:135753. doi: 10.1016/j.foodchem.2023.135753. Epub 2023 Feb 24.
Cuticular wax has been reported to play an essential role in resisting pathogens in various fruits. This study investigated the antifungal ability of the components in blueberry cuticular wax. We showed that the cuticular wax of blueberry inhibited the growth of Botrytis cinerea and ursolic acid (UA) was the key antifungal compound. UA inhibited B. cinerea growth in vitro and in vivo. Furthermore, UA increased extracellular conductivity and cellular leakage in B. cinerea, deformed the mycelial morphology, and destroyed cell ultrastructure. We also demonstrated that UA stimulated the accumulation of reactive oxygen species (ROS) and inactivated ROS scavenging enzymes. These results indicate that UA may exert antifungal effects against B. cinerea by disrupting cell membrane integrity. Thus, UA has significant potential as an agent for the control of gray mold in blueberry.
已有研究表明，表皮蜡质在各种水果抵抗病原菌的过程中发挥着重要作用。本研究调查了蓝莓表皮蜡质成分的抗真菌能力。结果表明，蓝莓表皮蜡质能够抑制灰葡萄孢的生长，其中熊果酸（UA）是关键的抗真菌化合物。UA 在体外和体内均能抑制灰葡萄孢的生长。此外，UA 增加了灰葡萄孢的细胞外电导率和细胞渗漏，使菌丝形态发生变形，并破坏了细胞超微结构。我们还证明，UA 能够刺激活性氧（ROS）的积累并使 ROS 清除酶失活。这些结果表明，UA 可能通过破坏细胞膜完整性对灰葡萄孢发挥抗真菌作用。因此，UA 作为蓝莓灰霉病防治剂具有重要的应用潜力。
