Ni Jie, Yu Lian, Li Fei, Li Yulin, Zhang Mengfei, Deng Yuping, Liu Xiaoling
Department of Food Science and Engineering, College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, Guangxi, P.R. China.
Guangxi Key Laboratory of Marine Environmental Science, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, 530007, P.R. China.
World J Microbiol Biotechnol. 2023 Mar 15;39(5):117. doi: 10.1007/s11274-023-03563-x.
Botrytis cinerea is listed among the most important fungal pathogens infecting strawberries. The use of biological control agents, such as Bacillus species, offers an alternative and effective way to reduce airborne pathogens. The aim of this research was to select the macrolactin R produced by Bacillus siamensis with potential for using as biological agents against the pathogenetic fungi (Botrytis cinerea) of strawberries, and to assess the mechanisms involved. Macrolactin R had significant inhibitory effects on spore germination, germ tube elongation, and mycelial growth of Botrytis cinerea. The MICs of macrolactin R inhibitions in vitro was 12.5 mg/L and The EC value of NJ08-3 to Botrytis cinerea spores and mycelial was 1.93 and 2.88 mg/L, respectively. Macrolactin R impacted the membrane structure of Botrytis cinerea, resulting in changes in membrane permeability and leakage of proteins and nucleic acids, then cell death. The application of the macrolactin R of Bacillus siamensis reduced the disease severity index of gray mold on strawberries. This study demonstrated that the production of macrolactin R produced by Bacillus siamensis are involved in the antifungal activity against Botrytis cinerea.
灰葡萄孢菌被列为感染草莓的最重要真菌病原体之一。使用生物防治剂，如芽孢杆菌属，为减少空气传播病原体提供了一种替代且有效的方法。本研究的目的是筛选暹罗芽孢杆菌产生的具有抗草莓致病真菌（灰葡萄孢菌）潜力的大环内酯R，并评估其作用机制。大环内酯R对灰葡萄孢菌的孢子萌发、芽管伸长和菌丝生长具有显著抑制作用。大环内酯R体外抑制的最低抑菌浓度为12.5mg/L，NJ08-3对灰葡萄孢菌孢子和菌丝体的有效浓度分别为1.93mg/L和2.88mg/L。大环内酯R影响灰葡萄孢菌的膜结构，导致膜通透性改变以及蛋白质和核酸泄漏，进而导致细胞死亡。暹罗芽孢杆菌大环内酯R的应用降低了草莓灰霉病的病情严重指数。本研究表明，暹罗芽孢杆菌产生的大环内酯R参与了对灰葡萄孢菌的抗真菌活性。
