通过比较健康年轻成年人摄入三文鱼与其分离营养素的总和，从食品基质角度阐释运动后肌原纤维蛋白合成的调控。
Underpinning the Food Matrix Regulation of Postexercise Myofibrillar Protein Synthesis by Comparing Salmon Ingestion With the Sum of Its Isolated Nutrients in Healthy Young Adults.
机构信息
Department of Kinesiology and Community Health, University of Illinois Urbana-Champaign, Urbana, IL, United States.
Division of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, IL, United States.
出版信息
J Nutr. 2023 May;153(5):1359-1372. doi: 10.1016/j.tjnut.2023.02.037. Epub 2023 Mar 2.
BACKGROUND
Protein is most commonly consumed as whole foods as opposed to single nutrients. However, the food matrix regulation of the postprandial muscle protein synthetic response has received little attention.
OBJECTIVES
The purpose of this study was to assess the effects of eating salmon (SAL) and of ingesting the same nutrients as an isolated mixture of crystalline amino acids and fish oil (ISO) on the stimulation of postexercise myofibrillar protein synthesis (MPS) and whole-body leucine oxidation rates in healthy young adults.
METHODS
Ten recreationally active adults (24 ± 4 y; 5 men, 5 women) performed an acute bout of resistance exercise, followed by the ingestion of SAL or ISO in a crossover fashion. Blood, breath, and muscle biopsies were collected at rest and after exercise during primed continuous infusions of L-[ring-H]phenylalanine and L-[1-C]leucine. All data are presented as means ± SD and/or mean differences (95% CIs).
RESULTS
Postprandial essential amino acid (EAA) concentrations peaked earlier (P = 0.024) in the ISO group than those in the SAL group. Postprandial leucine oxidation rates increased over time (P < 0.001) and peaked earlier in the ISO group (1.239 ± 0.321 nmol/kg/min; 63 ± 25 min) than those in the SAL group (1.230 ± 0.561 nmol/kg/min; 105 ± 20 min; P = 0.003). MPS rates for SAL (0.056 ± 0.022 %/h; P = 0.001) and ISO (0.046 ± 0.025 %/h; P = 0.025) were greater than the basal rates (0.020 ± 0.011 %/h) during the 0- to 5-h recovery period, with no differences between conditions (P = 0.308).
CONCLUSION
We showed that the postexercise ingestion of SAL or ISO stimulate postexercise MPS rates with no differences between the conditions. Thus, our results indicate that ingesting protein from SAL as a whole-food matrix is similarly anabolic to ISO in healthy young adults. This trial was registered at www.
CLINICALTRIALS
gov as NCT03870165.
背景
蛋白质通常作为完整的食物摄入，而不是单一的营养素。然而，食物基质对餐后肌肉蛋白质合成反应的调节却很少受到关注。
目的
本研究旨在评估食用三文鱼（SAL）和摄入结晶氨基酸和鱼油的混合物（ISO）对健康年轻成年人运动后肌原纤维蛋白合成（MPS）和全身亮氨酸氧化率的刺激作用。
方法
10 名有规律运动的成年人（24 ± 4 岁；5 男，5 女）进行了一次急性抗阻运动，随后以交叉方式摄入 SAL 或 ISO。在持续输注 L-[ring-H]苯丙氨酸和 L-[1-C]亮氨酸的基础上，在休息和运动后采集血液、呼吸和肌肉活检。所有数据均以平均值 ± SD 和/或平均值差异（95%可信区间）表示。
结果
餐后必需氨基酸（EAA）浓度在 ISO 组中更早达到峰值（P = 0.024）。亮氨酸氧化率随时间增加（P < 0.001），在 ISO 组中更早达到峰值（1.239 ± 0.321 nmol/kg/min；63 ± 25 min），而在 SAL 组中更早达到峰值（1.230 ± 0.561 nmol/kg/min；105 ± 20 min；P = 0.003）。SAL（0.056 ± 0.022 %/h；P = 0.001）和 ISO（0.046 ± 0.025 %/h；P = 0.025）的 MPS 率高于基础率（0.020 ± 0.011 %/h），在 0-5 小时的恢复期内，两种条件之间无差异（P = 0.308）。
结论
我们表明，运动后摄入 SAL 或 ISO 均可刺激运动后 MPS 率，且两种条件之间无差异。因此，我们的结果表明，在健康年轻成年人中，以完整食物基质摄入 SAL 中的蛋白质与 ISO 同样具有合成代谢作用。本试验在 www.clinicaltrials.gov 上注册，编号为 NCT03870165。
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