College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, PR China.
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Biotechnol Adv. 2023 May-Jun;64:108125. doi: 10.1016/j.biotechadv.2023.108125. Epub 2023 Mar 2.
As the two most widely used Kluyveromyces yeast, Kluyveromyces marxianus and K. lactis have gained increasing attention as microbial chassis in biocatalysts, biomanufacturing and the utilization of low-cost raw materials owing to their high suitability to these applications. However, due to slow progress in the development of molecular genetic manipulation tools and synthetic biology strategies, Kluyveromyces yeast cell factories as biological manufacturing platforms have not been fully developed. In this review, we provide a comprehensive overview of the attractive characteristics and applications of Kluyveromyces cell factories, with special emphasis on the development of molecular genetic manipulation tools and systems engineering strategies for synthetic biology. In addition, future avenues in the development of Kluyveromyces cell factories for the utilization of simple carbon compounds as substrates, the dynamic regulation of metabolic pathways, and for rapid directed evolution of robust strains are proposed. We expect that more synthetic systems, synthetic biology tools and metabolic engineering strategies will adapt to and optimize for Kluyveromyces cell factories to achieve green biofabrication of multiple products with higher efficiency.
作为两种应用最广泛的克鲁维酵母，马克斯克鲁维酵母和乳酸克鲁维酵母由于其在生物催化剂、生物制造和利用低成本原料方面的高度适用性，作为微生物底盘越来越受到关注。然而，由于分子遗传操作工具和合成生物学策略的发展缓慢，克鲁维酵母细胞工厂作为生物制造平台尚未得到充分发展。在这篇综述中，我们全面概述了克鲁维酵母细胞工厂的诱人特性和应用，特别强调了分子遗传操作工具和合成生物学系统工程策略的发展。此外，还提出了利用简单碳化合物作为底物、代谢途径的动态调控以及快速定向进化健壮菌株等方面的未来发展方向。我们期望更多的合成系统、合成生物学工具和代谢工程策略将适应和优化克鲁维酵母细胞工厂，以实现多种产品的高效绿色生物制造。
