Sato Keigo, Jinno Shinji, Nakamura Yoshitaka, Eto Shinichi, Inagaki Mizuho
Food Microbiology and Function Research Laboratory, Meiji Co. Ltd. 1-29-1 Nanakui, Hachioji, Tokyo 192-0919, Japan.
Food Microbiology and Function Research Laboratory, Meiji Co. Ltd. 1-29-1 Nanakui, Hachioji, Tokyo 192-0919, Japan.
J Dairy Sci. 2023 Apr;106(4):2261-2270. doi: 10.3168/jds.2022-22401. Epub 2023 Mar 2.
The anti-rotavirus components in breast milk and infant formulas play an important role in the prevention of rotavirus infection. The present study examined whether the levels of phospholipids and bovine lactadherin, which are the major components and proteins of the milk fat globule membrane complex, are useful indices of the anti-rotavirus activity of dairy ingredients used in infant formulas. We compared the anti-rotavirus activity of 2 types of dairy ingredients enriched in the milk fat globule membrane complex: high-fat whey protein concentrate (high-fat WPC) and butter milk powder (BMP), using 50% inhibition concentration (IC) and linear inhibition activity to determine levels of solid contents, total proteins, phospholipids, and bovine lactadherin. Here, we developed a quantification method using full-length isotope-labeled proteins to measure bovine lactadherin levels in these dairy ingredients. The evaluation of anti-rotavirus activity showed that the difference in IC was the smallest when the 2 dairy ingredients were compared at the bovine lactadherin level, among other indices in this study. Additionally, no significant difference was observed between the inhibition linearity of 2 dairy ingredients when evaluating only bovine lactadherin levels. These results indicated that the level of bovine lactadherin was more strongly associated with anti-rotavirus activity than the level of phospholipids. Our results suggest that bovine lactadherin levels can be used to estimate the anti-rotavirus activity of dairy ingredients and can be a criterion used in selecting ingredients for infant formulas.
母乳和婴儿配方奶粉中的抗轮状病毒成分在预防轮状病毒感染方面发挥着重要作用。本研究调查了作为乳脂肪球膜复合物主要成分和蛋白质的磷脂和牛乳糖黏附蛋白水平，是否可作为婴儿配方奶粉中乳制品成分抗轮状病毒活性的有用指标。我们使用50%抑制浓度（IC）和线性抑制活性来测定固体成分、总蛋白、磷脂和牛乳糖黏附蛋白的水平，比较了富含乳脂肪球膜复合物的两种乳制品成分的抗轮状病毒活性：高脂乳清蛋白浓缩物（高脂WPC）和酪乳粉（BMP）。在此，我们开发了一种使用全长同位素标记蛋白的定量方法，以测量这些乳制品成分中的牛乳糖黏附蛋白水平。抗轮状病毒活性评估表明，在本研究的其他指标中，当比较两种乳制品成分在牛乳糖黏附蛋白水平时，IC差异最小。此外，仅评估牛乳糖黏附蛋白水平时，两种乳制品成分的抑制线性之间未观察到显著差异。这些结果表明，牛乳糖黏附蛋白水平与抗轮状病毒活性的相关性比磷脂水平更强。我们的结果表明，牛乳糖黏附蛋白水平可用于估计乳制品成分的抗轮状病毒活性，并且可以作为选择婴儿配方奶粉成分的一个标准。
