Kvistgaard A S, Pallesen L T, Arias C F, López S, Petersen T E, Heegaard C W, Rasmussen J T
Protein Chemistry Laboratory, University of Aarhus, Denmark.
J Dairy Sci. 2004 Dec;87(12):4088-96. doi: 10.3168/jds.S0022-0302(04)73551-1.
Among etiologic agents, rotavirus is the major cause of severe dehydration diarrhea in infant mammals. In vitro and in vivo studies have indicated that the human milk-fat globule protein lactadherin inhibits rotavirus binding and protects breast-fed children against symptomatic rotavirus infection. The present work was conducted to evaluate the effect of lactadherin, along with some other milk proteins and fractions, on rotavirus infections in MA104 and Caco-2 cell lines. It is shown that human, and not bovine, lactadherin inhibits Wa rotavirus infection in vitro. Human lactadherin seems to act through a mechanism involving protein-virus interactions. The reason for the activity of human lactadherin is not clear, but it might lie within differences in the protein structure or the attached oligosaccharides. Likewise, in our hands, bovine lactoferrin did not show any suppressive activity against rotavirus. In contrast, MUC1 from bovine milk inhibits the neuraminidase-sensitive rotavirus RRV strain efficiently, whereas it has no effect on the neuraminidase-resistant Wa strain. Finally, a bovine macromolecular whey protein fraction turned out to have an efficient and versatile inhibitory activity against rotavirus.
在病原体中，轮状病毒是婴儿哺乳动物严重脱水腹泻的主要病因。体外和体内研究表明，人乳脂肪球蛋白乳黏附素可抑制轮状病毒结合，并保护母乳喂养的儿童免受有症状的轮状病毒感染。本研究旨在评估乳黏附素以及其他一些乳蛋白和组分对MA104和Caco-2细胞系中轮状病毒感染的影响。结果表明，人乳黏附素而非牛乳黏附素在体外可抑制Wa轮状病毒感染。人乳黏附素似乎通过涉及蛋白质-病毒相互作用的机制发挥作用。人乳黏附素具有活性的原因尚不清楚，但可能在于蛋白质结构或附着的寡糖的差异。同样，在我们的实验中，牛乳转铁蛋白对轮状病毒未显示出任何抑制活性。相反，牛乳中的MUC1可有效抑制对神经氨酸酶敏感的轮状病毒RRV株，而对神经氨酸酶抗性的Wa株则无作用。最后，一种牛乳大分子乳清蛋白组分对轮状病毒具有高效且通用的抑制活性。
