Ling Wei, Qin Yan-Hong, Huang Ding, Ming Ru-Hong, Tan Yong
Institute of Pharmacy, Guangxi University of Chinese Medicine Nanning 530000, China.
Institute of Pharmacy, Guangxi University of Chinese Medicine Nanning 530000, China Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine Nanning 530200, China.
Zhongguo Zhong Yao Za Zhi. 2023 Feb;48(4):930-938. doi: 10.19540/j.cnki.cjcmm.20221102.101.
The present study aimed to investigate the composition of the terpene synthase(TPS) gene family in Gynostemma pentaphyllum and its role in abiotic stresses. The G. pentaphyllum TPS gene family was identified and analyzed at the genome-wide level using bioinformatics analysis, and the expression patterns of these family members were analyzed in different tissues of G. pentaphyllum as well as under various abiotic stresses. The results showed that there were 24 TPS gene family members in G. pentaphyllum with protein lengths ranging from 294 to 842 aa. All of them were localized in the cytoplasm or chloroplasts and unevenly distributed on the 11 chromosomes of G. pentaphyllum. The results of the phylogenetic tree showed that the G. pentaphyllum TPS gene family members could be divided into five subfamilies. As revealed by the analysis of promoter cis-acting elements, TPS gene family members in G. pentaphyllum were predicted to respond to a variety of abiotic stresses such as salt, low temperature, and dark stress. The analysis of gene expression patterns in different tissues of G. pentaphyllum revealed that nine TPS genes were tissue-specific in expression. The qPCR results showed that GpTPS16, GpTPS17, and GpTPS21 responded to a variety of abiotic stresses. This study is expected to provide references in guiding the further exploration of the biological functions of G. pentaphyllum TPS genes under abiotic stresses.
本研究旨在探究绞股蓝萜类合酶（TPS）基因家族的组成及其在非生物胁迫中的作用。利用生物信息学分析在全基因组水平上对绞股蓝TPS基因家族进行了鉴定和分析，并分析了这些家族成员在绞股蓝不同组织以及各种非生物胁迫下的表达模式。结果表明，绞股蓝中有24个TPS基因家族成员，蛋白质长度在294至842个氨基酸之间。它们均定位于细胞质或叶绿体中，且在绞股蓝的11条染色体上分布不均。系统发育树结果表明，绞股蓝TPS基因家族成员可分为五个亚家族。通过对启动子顺式作用元件的分析发现，绞股蓝TPS基因家族成员预计对盐、低温和黑暗胁迫等多种非生物胁迫作出响应。对绞股蓝不同组织中基因表达模式的分析表明，9个TPS基因在表达上具有组织特异性。qPCR结果表明，GpTPS16、GpTPS17和GpTPS21对多种非生物胁迫有响应。本研究有望为进一步探索绞股蓝TPS基因在非生物胁迫下的生物学功能提供参考。
