Omer Asma B, Afzal Obaid, Altamimi Abdulmalik S A, Patil Shaktipal, AlGhamdi Shareefa A, Alghamdi Amira M, Alzarea Sami I, Almalki Waleed Hassan, Kazmi Imran
Department of Basic Health Sciences, Foundation Year for the Health Colleges, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
ACS Omega. 2023 Feb 16;8(8):8110-8118. doi: 10.1021/acsomega.2c08277. eCollection 2023 Feb 28.
Streptozotocin (STZ) impairs memory in rats through altering the central nervous systems (CNS) as a result of impaired cholinergic dysfunction, oxidative stress, persistent hyperglycemia, and alterations in the glucagon-like peptide (GLP). In this model cholinergic agonist, antioxidant and antihyperglycemic treatment has been shown to have positive effects. Barbaloin has a variety of pharmacological effects. However, there is no evidence on how barbaloin improves memory dysfunction caused by STZ. Thus, we examined its effectiveness against cognitive damage caused by STZ at a dose of 60 mg/kg i.p. in Wistar rats. Blood glucose levels (BGL) and body weight (BW) were assessed. To assess learning and memory skills, the Y-maze test and Morris water maze (MWM) test were utilized. Superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) as oxidative stress markers were regulated to reverse the cognitive deterioration, and choline-acetyltransferase (ChAT) and acetyl-cholinesterase (AChE) as indicators of cholinergic dysfunction, nuclear factor kappa-B (NF-κB), IL-1β (interleukin-1β), IL-6, and tumor necrosis factor-α (TNF-α) contents were used. Barbaloin treatment thereby significantly decreased the BW and learning and memory capacities, resulting in substantial behavioral improvement in the Y-maze and MWM test. BGL, SOD, CAT, MDA, GSH, AChE, ChAT, NF-κB, IL-6, TNF-α, and IL-1β levels were also altered. In conclusion, the findings revealed that barbaloin had a protective impact against cognitive dysfunction caused by STZ.
链脲佐菌素（STZ）通过改变中枢神经系统（CNS）损害大鼠记忆，这是由于胆碱能功能障碍、氧化应激、持续性高血糖以及胰高血糖素样肽（GLP）改变所致。在该模型中，胆碱能激动剂、抗氧化剂和抗高血糖治疗已显示出积极效果。芦荟苷具有多种药理作用。然而，尚无证据表明芦荟苷如何改善由STZ引起的记忆功能障碍。因此，我们在Wistar大鼠中以60mg/kg腹腔注射剂量研究了其对STZ所致认知损伤的有效性。评估了血糖水平（BGL）和体重（BW）。为评估学习和记忆技能，采用了Y迷宫试验和莫里斯水迷宫（MWM）试验。调节超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）和谷胱甘肽（GSH）作为氧化应激标志物以逆转认知恶化，并使用胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）作为胆碱能功能障碍指标、核因子κB（NF-κB）、白细胞介素-1β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）含量。芦荟苷治疗从而显著降低了体重和学习及记忆能力，在Y迷宫和MWM试验中导致行为有实质性改善。BGL、SOD、CAT、MDA、GSH、AChE、ChAT、NF-κB、IL-6、TNF-α和IL-1β水平也发生了改变。总之，研究结果表明芦荟苷对STZ所致认知功能障碍具有保护作用。
