Motallebzadeh Elham, Aghighi Fatemeh, Vakili Zarichehr, Talaei Sayyed Alireza, Mohseni Mehran
Physiology Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, I.R. Iran.
Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, I.R. Iran.
Res Pharm Sci. 2023 Jan 19;18(2):202-209. doi: 10.4103/1735-5362.367798. eCollection 2023 Apr.
Alpha-lipoic acid (ALA) is an antioxidant with radioprotective properties. We designed the current work to assess the neuroprotective function of ALA in the presence of oxidative stress induced by radiation in the brainstem of rats.
Whole-brain radiations (X-rays) was given at a single dose of 25 Gy with or without pretreatment with ALA (200 mg/kg BW). Eighty rats were categorized into four groups: vehicle control (VC), ALA, radiation-only (RAD), and radiation + ALA (RAL). The rats were given ALA intraperitoneally 1 h before radiation and killed following 6 h, thereafter superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and total antioxidant capacity (TAC) in the brainstem were measured. Furthermore, a pathological examination was carried out after 24 h, 72 h, and five days to determine tissue damage.
FINDINGS/RESULTS: The findings indicated that MDA levels in the brainstem were 46.29 ± 1.64 μM in the RAD group and decreased in the VC group (31.66 ± 1.72 μM). ALA pretreatment reduced MDA levels while simultaneously increasing SOD and CAT activity and TAC levels (60.26 ± 5.47 U/mL, 71.73 ± 2.88 U/mL, and 227.31 ± 9.40 mol/L, respectively). The greatest pathological changes in the rat's brainstems were seen in RAD animals compared to the VC group after 24 h, 72 h, and 5 days. As a result, karyorrhexis, pyknosis, vacuolization, and Rosenthal fibers vanished in the RAL group in three periods.
ALA exhibited substantial neuroprotectivity following radiation-induced brainstem damage.
α-硫辛酸（ALA）是一种具有辐射防护特性的抗氧化剂。我们开展本研究以评估在大鼠脑干中由辐射诱导的氧化应激存在情况下ALA的神经保护功能。
给予单次剂量25 Gy的全脑辐射（X射线），同时或不给予ALA预处理（200 mg/kg体重）。80只大鼠被分为四组：溶剂对照组（VC）、ALA组、单纯辐射组（RAD）和辐射+ALA组（RAL）。在辐射前1小时给大鼠腹腔注射ALA，并在6小时后处死，然后测量脑干中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和总抗氧化能力（TAC）。此外，在24小时、72小时和五天后进行病理检查以确定组织损伤情况。
结果表明，RAD组脑干中的MDA水平为46.29±1.64μM，而VC组有所降低（31.66±1.72μM）。ALA预处理降低了MDA水平，同时提高了SOD和CAT活性以及TAC水平（分别为60.26±5.47 U/mL、71.73±2.88 U/mL和227.31±9.40 mol/L）。与VC组相比，在24小时、72小时和5天后，RAD组大鼠脑干中的病理变化最为明显。因此，在三个时间段中，RAL组的核固缩、核碎裂、空泡化和罗森塔尔纤维消失。
ALA在辐射诱导的脑干损伤后表现出显著的神经保护作用。
