Jafarpour Seyed Masoud, Safaei Mehdi, Mohseni Mehran, Salimian Morteza, Aliasgharzadeh Akbar, Farhood Bagher
Department of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Department of Medical Laboratory, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Indian J Nucl Med. 2018 Apr-Jun;33(2):99-104. doi: 10.4103/ijnm.IJNM_158_17.
Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes.
First, 6-mL blood samples were taken from each of the five volunteers. After 1 h of preincubation with the antioxidants, a total of 20 μCi I-131/2 mL (blood + NaCl) was added to each sample, and then, the samples were reincubated for 1 h. Lymphocytes were separated and the mean DSB levels were measured for each sample through γ-H2AX assay to evaluate the effects of antioxidants.
After 1-h incubation with I-131, the DSBs increased by 102.9% compared to the control group (0.343 vs. 0.169 DSB/cell; = 0.00). Furthermore, compared to the control + I-131 group, curcumin and trehalose reduced the DSBs by 42% and 38%, respectively. There was a significant decrement ( = 0.00) in the levels of DSBs of the curcumin + I-131 and trehalose + I-131 subgroups compared to the control + I-131 subgroup. Furthermore, there was no significant relationship between the radioprotective effect of curcumin and trehalose ( = 0.95).
The use of curcumin and trehalose as antioxidant can reduce the numbers of DSBs caused by I-131. Meanwhile, the radioprotective effect of curcumin was more than trehalose.
在过去几十年中，甲状腺癌发病率一直在快速上升。放射性碘-131（I-131）作为一种合适的治疗方式，目前被用于癌症和甲状腺功能亢进症的治疗。这种放射性示踪剂被认为是导致非靶细胞和组织中DNA氧化损伤的一个原因。本研究旨在探讨姜黄素和海藻糖对I-131所致人淋巴细胞DNA双链断裂（DSB）水平的影响。
首先，从5名志愿者中每人采集6 mL血液样本。在与抗氧化剂预孵育1小时后，向每个样本中加入总计20 μCi I-131/2 mL（血液+氯化钠），然后将样本再孵育1小时。分离淋巴细胞，并通过γ-H2AX检测法测量每个样本的平均DSB水平，以评估抗氧化剂的效果。
与I-131孵育1小时后，DSB较对照组增加了102.9%（0.343对0.169 DSB/细胞；P = 0.00）。此外，与对照组+I-131组相比，姜黄素和海藻糖分别使DSB减少了42%和38%。与对照组+I-131亚组相比，姜黄素+I-131和海藻糖+I-131亚组的DSB水平有显著下降（P = 0.00）。此外，姜黄素和海藻糖的辐射防护作用之间无显著相关性（P = 0.95）。
姜黄素和海藻糖作为抗氧化剂可减少I-131所致的DSB数量。同时，姜黄素的辐射防护作用强于海藻糖。
