Rezende Mires de Carvalho Rodrigo, Silva Dias Carla, Nogueira Paz Lucas, Melo de Lima Fires Thainá, Pereira Figueira Cláudio, Araújo Damasceno Karine, Hanzen Pinna Melissa
Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Salvador, Bahia, Brazil.
Postgraduate Program in Clinic and Surgery of Domestic Carnivores - Federal University of Bahia, Salvador, Bahia, Brazil.
Heliyon. 2023 Feb 16;9(3):e13802. doi: 10.1016/j.heliyon.2023.e13802. eCollection 2023 Mar.
is a biofilm-forming pathogen, however, there are few data involving Brazilian strains isolated from dogs and their antimicrobial sensitivity in planktonic and biofilm forms. The potential for biofilm formation and antimicrobial resistance in naturally infected dogs is a fundamental approach towards disease epidemiology and the establishment of consistent prophylaxis and control measures. The objective of this study was to evaluate biofilm formation of a reference strain (, sv. Copenhageni L1 130 - L20) and of isolated from dogs (C20, C29, C51, C82), with subsequent evaluation of antimicrobial susceptibility in planktonic and biofilm forms. The semi quantification of biofilm production revealed a dynamic process of development over time, with mature biofilm formation early on the seventh day of incubation. All strains were efficient for biofilm formation and, in this form, they were considerably more resistant compared to their planktonic form, with MIC of 1600 μg/mL for amoxicillin, 800 μg/mL for ampicillin, and >1600 μg/mL for doxycycline and ciprofloxacin. The strains studies were isolated on naturally infected dogs that might act as reservoirs and sentinels for human infections. The potential to antimicrobial resistance together with the close relation between dogs and humans indicates the need for greater actions on disease control and surveillance. Moreover, biofilm formation may contribute to the persistence of in the host and these animals can act as chronic carriers, disseminating the agent in the environment.
是一种形成生物膜的病原体，然而，关于从狗身上分离出的巴西菌株及其浮游和生物膜形式的抗菌敏感性的数据很少。自然感染犬只中生物膜形成和抗菌耐药性的潜力是疾病流行病学以及建立一致的预防和控制措施的基本方法。本研究的目的是评估参考菌株（，血清型哥本哈根i L1 130 - L20）和从狗身上分离出的菌株（C20、C29、C51、C82）的生物膜形成情况，随后评估其浮游和生物膜形式的抗菌敏感性。生物膜产生的半定量显示了一个随时间发展的动态过程，在培养的第七天早期形成成熟生物膜。所有菌株都能有效地形成生物膜，并且在这种形式下，它们比浮游形式具有更强的耐药性，阿莫西林的最低抑菌浓度为1600μg/mL，氨苄西林为800μg/mL，强力霉素和环丙沙星大于1600μg/mL。所研究的菌株是从自然感染的狗身上分离出来的，这些狗可能是人类感染的储存宿主和哨兵。抗菌耐药性的潜力以及狗与人类之间的密切关系表明需要在疾病控制和监测方面采取更多行动。此外，生物膜的形成可能有助于病原体在宿主体内持续存在，并且这些动物可以作为慢性携带者，在环境中传播病原体。
