El-Mekkawy Rasha M, Hamour Noura E, Hassanein Wesam A, Allam Ayman A
Department of Botany and Microbiology, Faculty of Science, Zagazig University, 44519, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Egypt.
Saudi J Biol Sci. 2023 Apr;30(4):103595. doi: 10.1016/j.sjbs.2023.103595. Epub 2023 Feb 21.
Different strategies have been approved for controlling extended-spectrum βeta lactamase (ESBL) producing uropathogenic bacteria. The antibacterial activity of Lactic acid bacteria (LAB) is an effective strategy due to its probiotic characteristics and beneficial effects on human health. The antibiotic susceptibility test, disk diffusion method, and double disc synergy test indicated that five enteric uropathogenic isolates were ESBL producers during the present study. They recorded diameters of inhibition zones as ≤ 18, ≤ 8, ≤ 19, and ≤ 8 mm against cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), and ceftriaxone (CRO). Genotypically, genes are the most common, with (100 %) occurrence in all the five enteric tested uropathogens, followed by and genes (60 %). In addition, out of 10 LAB isolates from dairy products, the CFS of isolate no. K3 had high antibacterial activity against the tested ESBLs, especially no. U60, with a MIC of 600 µl. Additionally, the MIC and sub-MIC of K3 CFS inhibited the production of antibiotic-resistant genes of U60. Analyzing the 16S rRNA sequence confirmed that the most potent ESBL-producing bacteria (U60) and LAB (K3) isolates were identified as U60.1 and K3 with accession numbers MW173246 and MW173299.1, respectively, in GenBank.
已经批准了不同的策略来控制产超广谱β-内酰胺酶（ESBL）的尿路致病性细菌。乳酸菌（LAB）的抗菌活性是一种有效的策略，因为其具有益生菌特性且对人类健康有益。抗生素敏感性试验、纸片扩散法和双纸片协同试验表明，在本研究中，五株肠道尿路致病性分离株为ESBL产生菌。它们对头孢噻肟（CTX）、头孢他啶（CAZ）、氨曲南（ATM）和头孢曲松（CRO）的抑菌圈直径分别≤18、≤8、≤19和≤8毫米。从基因类型来看，某些基因最为常见，在所有五株受试肠道尿路致病菌中出现率为100%，其次是另外两种基因，出现率为60%。此外，在从乳制品中分离出的10株LAB中，菌株K3的无细胞上清液（CFS）对受试ESBLs具有高抗菌活性，尤其是对菌株U60，其最低抑菌浓度（MIC）为600微升。此外，K3 CFS的MIC和亚MIC抑制了U60抗生素耐药相关基因的产生。对16S rRNA序列进行分析证实，最具强效的产ESBL细菌（U60）和LAB（K3）分离株在GenBank中分别被鉴定为肺炎克雷伯菌U60.1和植物乳杆菌K3，登录号分别为MW173246和MW173299.1。
