Abbas Ali, Anwar Farooq, Ahmad Naveed, Rehman Afifa Tur, Mohammed Osama A, Abdel-Reheim Mustafa Ahmed, Iqbal Munawar, Iqbal Shahid, Nazir Arif
Institute of Chemistry, University of Sargodha, Sargodha, 41000, Pakistan.
Department of Chemistry, Govt. Islamia Graduate College, Chiniot, Pakistan.
Heliyon. 2024 Jul 26;10(16):e35282. doi: 10.1016/j.heliyon.2024.e35282. eCollection 2024 Aug 30.
This study reports the comparative evaluation of yield, physico-chemical composition and biological attributes (antioxidant activity, antimicrobial activity, biofilm inhibition and hemolytic activity) of peppermint ( L.) essential oil (EO) obtained by hydro-distillation (HD) and supercritical fluid (CO) extraction (SCFE) methods. The yield (%) of EO obtained by HD (0.20 %) was significantly ( < 0.05) higher than that of SCFE (0.13 %) while the variation in the physical parameters like solubility, color, density (at 25 °C) and refractive index (at 25 °C) was not significant between the tested oils. The data of chemical compositional analysis revealed that menthol was the key component in the EO obtained by HD (52.85 %) and SCFE (45.51 %), followed by menthone [HD (25.93 %) and SCFE (27.3 %)] and eucalyptol [HD (8.59 %); SCFE (8.92 %)]. The EO extracted with supercritical fluid (SCFE-EO) exhibited superior ( < 0.05) DPPH free radical inhibition potential (52 %) with an IC value of 15.65 μg/mL and reducing power compared to that of HD-EO. The highest antimicrobial activity was exhibited by SCFE-EO against with an inhibition zone of 18.00 mm (MIC value of 86 μg/mL). The results of biofilm inhibition and hemolytic activity revealed that the SCFE method was superior to recover high quality EO in comparison to the HD method. The peppermint EO obtained by SCFE, owing to potent bioactive components, can be a potential candidate to develop nutra-pharmaceuticals.
本研究报告了通过水蒸馏（HD）和超临界流体（CO₂）萃取（SCFE）方法获得的薄荷（Mentha piperita L.）精油（EO）在产量、理化成分和生物学特性（抗氧化活性、抗菌活性、生物膜抑制和溶血活性）方面的比较评估。HD法获得的EO产量（0.20%）显著（P < 0.05）高于SCFE法（0.13%），而在溶解度、颜色、密度（25℃）和折射率（25℃）等物理参数方面，受试精油之间的差异不显著。化学成分分析数据显示，薄荷醇是HD法（52.85%）和SCFE法（45.51%）获得的EO中的关键成分，其次是薄荷酮[HD法（25.93%）和SCFE法（27.3%）]和桉叶油素[HD法（8.59%）；SCFE法（8.92%）]。与HD-EO相比，超临界流体萃取的EO（SCFE-EO）表现出更高（P < 0.05）的DPPH自由基抑制潜力（52%），IC₅₀值为15.65μg/mL，且还原能力更强。SCFE-EO对金黄色葡萄球菌表现出最高的抗菌活性，抑菌圈为18.00mm（MIC值为86μg/mL）。生物膜抑制和溶血活性结果表明，与HD法相比，SCFE法在回收高质量EO方面更具优势。通过SCFE获得的薄荷EO由于含有有效的生物活性成分，可能是开发营养药物的潜在候选物。
