van der Nest Ariska, Wingfield Michael J, Sadiković Dušan, Mullett Martin S, Marçais Benoit, Queloz Valentin, Adamčíková Katarina, Davydenko Kateryna, Barnes Irene
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa.
Slovenian Forestry Institute, Ljubljana, Slovenia.
Front Genet. 2023 Feb 16;14:1103331. doi: 10.3389/fgene.2023.1103331. eCollection 2023.
Dothistroma needle blight (DNB) is an important disease of species that can be caused by one of two distinct but closely related pathogens; and . has a wide geographic distribution and is relatively well-known. In contrast, is known only from the United States and Europe, and there is a distinct lack of knowledge regarding its population structure and genetic diversity. The recent development of 16 microsatellite markers for provided an opportunity to investigate the diversity, structure, and mode of reproduction for populations collected over a period of 12 years, on eight different hosts in Europe. In total, 345 isolates from Belgium, the Czech Republic, France, Hungary, Romania, Western Russia, Serbia, Slovakia, Slovenia, Spain, Switzerland, and Ukraine were screened using microsatellite and species-specific mating type markers. A total of 109 unique multilocus haplotypes were identified and structure analyses suggested that the populations are influenced by location rather than host species. Populations from France and Spain displayed the highest levels of genetic diversity followed by the population in Ukraine. Both mating types were detected in most countries, with the exception of Hungary, Russia and Slovenia. Evidence for sexual recombination was supported only in the population from Spain. The observed population structure and several shared haplotypes between non-bordering countries provides good evidence that the movement of in Europe has been strongly influenced by human activity in Europe.
散斑壳针孢叶枯病（DNB）是一种重要的病害，可由两种不同但密切相关的病原体之一引起；[病原体名称1]和[病原体名称2]。[病原体名称1]分布广泛且相对广为人知。相比之下，[病原体名称2]仅在美国和欧洲被发现，并且对其种群结构和遗传多样性明显缺乏了解。最近为[病原体名称2]开发的16个微卫星标记提供了一个机会，来研究在欧洲12年期间从8种不同寄主上采集的种群的多样性、结构和繁殖方式。总共使用微卫星和物种特异性交配型标记对来自比利时、捷克共和国、法国、匈牙利、罗马尼亚、俄罗斯西部、塞尔维亚、斯洛伐克、斯洛文尼亚、西班牙、瑞士和乌克兰的345个分离株进行了筛选。共鉴定出109种独特的多位点单倍型，结构分析表明种群受地理位置而非寄主物种的影响。法国和西班牙的种群显示出最高水平的遗传多样性，其次是乌克兰的种群。除匈牙利、俄罗斯和斯洛文尼亚外，在大多数国家都检测到了两种交配型。仅在西班牙的种群中支持有性重组的证据。观察到的种群结构以及非接壤国家之间的几种共享单倍型提供了充分的证据，表明[病原体名称2]在欧洲的传播受到欧洲人类活动的强烈影响。
