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洗必泰/草药配方在减少超声洁治产生的气溶胶中微生物方面的疗效——一项双盲随机对照试验。

Efficacy of chlorhexidine/herbal formulation for microbial reduction in aerosol generated following ultrasonic scaling - A double-blinded randomized controlled trial.

作者信息

Ashokkumar Lekha, Lavu Vamsi, Palraj Kennedy Kumar, Rao Suresh Ranga, Balaji Subbusamy Kanakasabapathy

机构信息

Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

出版信息

J Indian Soc Periodontol. 2023 Jan-Feb;27(1):82-86. doi: 10.4103/jisp.jisp_478_21. Epub 2023 Jan 3.

Abstract

BACKGROUND

Ultrasonic scaling is a potential source of aerosol contamination in dental clinics. The two primary sources of microbial load in aerosols are from the oral cavity and dental unit water line. Literature evidence suggest that the use of preprocedural mouth rinse reduce the bacterial load in aerosol generated during ultrasonic scaling.

AIM

The aim of the study is to assess the comparative efficacy of reduction in viable bacteria in the aerosol at patient's chest area, doctor's mask area and two feet beside the patient following use of chlorhexidine/herbal formulation diluted in the water source by a randomized controlled clinical trial.

MATERIALS AND METHODS

Forty-five subjects (with chronic gingivitis) were matched for age, gender, and gingival index score. The subjects were randomized and received ultrasonic scaling with distilled water (control)/chlorhexidine (tTest)/herbal formulation (test). Aerosol produced during scaling was collected at patient's chest area, doctor's mask area, two feet beside the patient on blood agar plates, which were incubated at 37°C for 48 h and total colony forming units (CFUs) were counted.

RESULTS

A significant reduction in the total CFUs' counts was observed at all the three sites sampled in test groups (chlorhexidine group and herbal formulation group) as compared to control ( < 0.01).

CONCLUSION

The addition of antiseptic agents to the water source contributed to a significant reduction of the cultivable microbial counts in the aerosol and hence can be used to reduce the risk of cross-infection during ultrasonic scaling.

摘要

背景

超声洁牙是牙科诊所气溶胶污染的一个潜在来源。气溶胶中微生物负荷的两个主要来源是口腔和牙科设备的水线。文献证据表明,术前使用漱口水可减少超声洁牙过程中产生的气溶胶中的细菌负荷。

目的

本研究的目的是通过一项随机对照临床试验,评估在水源中稀释洗必泰/草药制剂后,患者胸部区域、医生口罩区域和患者旁边两英尺处的气溶胶中活菌减少的相对疗效。

材料与方法

45名(患有慢性牙龈炎)受试者按年龄、性别和牙龈指数评分进行匹配。受试者被随机分组,并接受用蒸馏水(对照组)/洗必泰(t检验组)/草药制剂(试验组)进行超声洁牙。洁牙过程中产生的气溶胶在患者胸部区域、医生口罩区域、患者旁边两英尺处收集到血琼脂平板上,于37°C孵育48小时,然后计算总菌落形成单位(CFU)。

结果

与对照组相比,试验组(洗必泰组和草药制剂组)在所有三个采样部位的总CFU计数均显著降低(<0.01)。

结论

在水源中添加抗菌剂有助于显著降低气溶胶中可培养的微生物数量,因此可用于降低超声洁牙过程中的交叉感染风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8a9/9979815/ec3c7a40e0d7/JISP-27-82-g001.jpg

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