Agahi Raha Habib, Hashemipour Maryam Alsadat, Kalantari Mahsa, Ayatollah-Mosavi Amin, Aghassi Hossein, Nassab Amir Hossein Gandjalikhan
Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Oral and Maxillofacial Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
Dent Res J (Isfahan). 2014 May;11(3):351-6.
It is known that dental unit waterline can be a source of infection. The aim of this study was to evaluate the efficacy of a mouthwash, chlorhexidine, in controlling microbial and fungal contamination of dental unit waterlines.
In the present experimental study, the water in high-speed handpieces and air/water syringes of 35 dental units in a dental school was investigated microbiologically. Five of the units and one tap water served as controls; 100-200-mL water samples were collected aseptically in sterile containers in the morning after a 2-min purge. Water reservoir bottles were emptied and 50 mL of 0.2% chlorhexidine mouthwash was introduced into the tank. Then the water syringe was used to flush the waterline until the pink-colored chlorhexidine was observed to flow from the water syringe. Before the next day's session and before the students used the unit, two water samples from the water syringe and water turbine was collected. The samples were transferred to the laboratory. After 48 h at 37°C, the microbial colonies were counted. The number of these colonies was evaluated using colony forming unit CFU. Data were analyzed with Mann - Whitney U test and SPSS 13.5 statistical program. The statistical significance was defined at P ≤ 0.05.
All 35 units were contaminated before chlorhexidine use; no contamination was detected after adding chlorhexidine to the waterlines of the units. After week 1, 28 of the 30 treated dental unit waterlines (DUWLs) had values of CFU/mL less than 200.
The present study showed that the use of chlorhexidine could reduce microbial counts in dental unit waterlines.
已知牙科设备水线可能是感染源。本研究的目的是评估洗必泰漱口水在控制牙科设备水线微生物和真菌污染方面的效果。
在本实验研究中，对一所牙科学院35台牙科设备的高速手机和空气/水注射器中的水进行了微生物学调查。其中5台设备和一份自来水作为对照；在早上经过2分钟冲洗后，无菌采集100 - 200毫升水样至无菌容器中。清空储水瓶，向水箱中加入50毫升0.2%的洗必泰漱口水。然后使用水注射器冲洗水线，直到观察到粉红色的洗必泰从水注射器中流出。在第二天课程开始前以及学生使用设备前，从水注射器和水涡轮收集两份水样。将样本转移至实验室。在37°C下培养48小时后，对微生物菌落进行计数。使用菌落形成单位CFU评估这些菌落的数量。数据采用曼 - 惠特尼U检验和SPSS 13.5统计程序进行分析。统计学显著性定义为P≤0.05。
在使用洗必泰之前，所有35台设备均被污染；在向设备水线中添加洗必泰后，未检测到污染。第1周后，30条经处理的牙科设备水线（DUWLs）中有28条的CFU/mL值低于200。
本研究表明，使用洗必泰可减少牙科设备水线中的微生物数量。
