Long Kai, Liu Jing, Shen Shuhao, Thong Mark, Wang Deyun, Chen Nanguang
Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, S117583, Singapore.
Department of Otolaryngology, Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore 119228, Singapore.
Comput Struct Biotechnol J. 2023 Feb 20;21:1661-1669. doi: 10.1016/j.csbj.2023.02.036. eCollection 2023.
Mucociliary clearance is an important innate defense mechanism predominantly mediated by ciliated cells in the upper respiratory tract. Ciliary motility on the respiratory epithelium surface and mucus pathogen trapping assist in maintaining healthy airways. Optical imaging methods have been used to obtain several indicators for assessing ciliary movement. Light-sheet laser speckle imaging (LSH-LSI) is a label-free and non-invasive optical technique for three-dimensional and quantitative mapping of velocities of microscopic scatterers. Here, we propose to use an inverted LSH-LSI platform to study cilia motility. We have experimentally confirmed that LSH-LSI can reliably measure the ciliary beating frequency and has the potential to provide many additional quantitative indicators for characterizing the ciliary beating pattern without labeling. For example, the asymmetry between the power stroke and the recovery stroke is apparent in the local velocity waveform. PIV (particle imaging velocimetry) analysis of laser speckle data could determine the cilia motion directions in different phases.
黏液纤毛清除是一种重要的先天性防御机制，主要由上呼吸道的纤毛细胞介导。呼吸道上皮表面的纤毛运动和黏液对病原体的捕获有助于维持气道健康。光学成像方法已被用于获取评估纤毛运动的多个指标。光片激光散斑成像（LSH-LSI）是一种用于对微观散射体速度进行三维定量映射的无标记、非侵入性光学技术。在此，我们提议使用倒置的LSH-LSI平台来研究纤毛运动。我们通过实验证实，LSH-LSI能够可靠地测量纤毛摆动频率，并且有潜力在不进行标记的情况下提供许多额外的定量指标来表征纤毛摆动模式。例如，在局部速度波形中，动力冲程和恢复冲程之间的不对称是明显的。对激光散斑数据进行粒子图像测速（PIV）分析可以确定不同阶段的纤毛运动方向。
