Remediation of zearalenone mycotoxin contamination in rumen fluid by phytochemical compounds of .

BACKGROUND

Zearalenone (ZEA), which is one of the most prevalent wheat and corn seeds mycotoxins causes acute and chronic toxicities in ruminants, poultry, and aquatic animals. Among commercial toxin binders, only a few active charcoals have the significant ability to adsorb ZEA contamination; nevertheless, active charcoal is not considered a sound additive by the feed industry.

AIMS

This study aimed to screen and identify the ZEA-degradation compounds of the (Shirazi thyme) in the cattle rumen fluid.

METHODS

In this investigation, essential oil and different extracts (-hexane, ethyl acetate, and methanol) of the aerial part of Shirazi thyme (at three concentrations of 0.5, 1, and 2 mg/ml) were screened to reduce ZEA contamination conditions (2 µg/ml) in rumen fluid. ZEA-content was analyzed by high-performance liquid chromatography (HPLC) with a fluorescence detector. In addition, Shirazi thyme phytochemical compounds responsible for eliminating ZEA were localized by HPLC-based activity profiling and then identified by mass spectrometry (LC-MS).

RESULTS

Both -hexane and methanol extracts of , considerably remediated ZEA (63-78%) from rumen fluid. According to HPLC-based activity profiling of extract and LC-MS analysis, two triterpene compounds, including ursolic and oleanolic acids were introduced as ZEA degradation agents.

CONCLUSION

could be recommended as a new botanical source, and ursolic and oleanolic acids could be introduced as new phytochemical compounds that degrade ZEA.