Sevastre Ani-Simona, Baloi Carina, Alexandru Oana, Tataranu Ligia Gabriela, Popescu Oana Stefana, Dricu Anica
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Str. Petru Rares nr. 2-4, 200349 Craiova, Romania.
Department of Biochemistry, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Str. Petru Rares nr. 2-4, 200349 Craiova, Romania.
Saudi J Biol Sci. 2023 Mar;30(3):103599. doi: 10.1016/j.sjbs.2023.103599. Epub 2023 Feb 14.
Despite the multidisciplinary standard treatment of glioblastoma (GB) consisting of maximal surgical resection, followed by radiotherapy (RT) plus concomitant chemotherapy with temozolomide (TMZ), the majority of patients experience tumor progression and almost universal mortality. In recent years, efforts have been made to create new agents for GB treatment, of which azo-dyes proved to be potential candidates, showing antiproliferative effects by inducing apoptosis and by inhibiting different signaling pathways. In this study we evaluated the antiproliferative the effect of six azo-dyes and TMZ on a low passage human GB cell line using MTT assay. We found that all compounds proved antiproliferative properties on GB cells. At equimolar concentrations azo-dyes induced more cytotoxic effect than TMZ. We found that Methyl Orange required the lowest IC for 3 days of treatment (26.4684 μM), whilst for 7 days of treatment, two azo dyes proved to have the highest potency: Methyl Orange IC = 13.8808 μM and Sudan I IC = 12.4829 μM. The highest IC was determined for TMZ under both experimental situations. Conclusions: Our research represents a novelty, by offering unique valuable data regarding the azo-dye cyototoxic effects in high grade brain tumors. This study may focus the attention on azo-dye agents that may represent an insufficient exploited source of agents for cancer treatment.
尽管胶质母细胞瘤（GB）的多学科标准治疗包括最大程度的手术切除，随后进行放疗（RT）并同时使用替莫唑胺（TMZ）进行化疗，但大多数患者仍会出现肿瘤进展，几乎无一例外最终死亡。近年来，人们致力于研发用于治疗GB的新型药物，其中偶氮染料被证明是潜在的候选药物，通过诱导细胞凋亡和抑制不同信号通路显示出抗增殖作用。在本研究中，我们使用MTT法评估了六种偶氮染料和TMZ对低传代人GB细胞系的抗增殖作用。我们发现所有化合物对GB细胞均具有抗增殖特性。在等摩尔浓度下，偶氮染料比TMZ诱导出更强的细胞毒性作用。我们发现，甲基橙在处理3天时所需的最低半数抑制浓度（IC）为26.4684μM，而在处理7天时，两种偶氮染料显示出最高的效力：甲基橙的IC = 13.880８μM，苏丹红Ｉ的IC = 12.4829μM。在两种实验情况下，TMZ的IC最高。结论：我们的研究具有创新性，提供了关于偶氮染料在高级别脑肿瘤中的细胞毒性作用的独特有价值的数据。本研究可能会使人们将注意力集中在偶氮染料药物上，这些药物可能是尚未得到充分开发的癌症治疗药物来源。
