Costachi Alexandra, Cioc Cătălina Elena, Buteică Sandra Alice, Tache Daniela Elise, Artene Ştefan-Alexandru, Sevastre Ani-Simona, Alexandru Oana, Tătăranu Ligia Gabriela, Popescu Ştefana Oana, Dricu Anica
University of Medicine and Pharmacy of Craiova, Romania.
"Bagdasar-Arseni" Emergency Clinical Hospital, Neurosurgery Department, Bucharest, Romania.
Curr Health Sci J. 2021 Jul-Sep;47(3):412-419. doi: 10.12865/CHSJ.47.03.12. Epub 2021 Sep 30.
The central nervous system tumors are the most common solid tumors in adults.. Unlike other types of cancers, brain cancer is much difficult to treat because of the blood-brain barrier (BBB) that prevents drug substances from crossing it and accessing the brain. Different types of methods to overcome BBB have been used in vivo and in vitro, of which the use of nanoparticle-mediated delivery of therapeutic drugs is particularly promising. In the present study, we used iron oxide magnetic nanoparticles (NPs) as carrier system for helianthin (He/NPs) to treat cancer cells derived from glioblastoma. An early passage cell cultures (GB1B), established in our laboratory from tissue obtained from a patient diagnosed with glioblastoma, was used. The cells were treated with different concentrations of NPs or HeNPs and then cell proliferation was measured at 24, 48 and 72 hours. Our results showed that the treatment with NPs was well tolerated by glioblastoma cells, the viability of the cells increased very slightly after the treatment. Furthermore, we demonstrated that helianthin loaded Fe3O4 magnetic nanoparticles induced cytotoxicity in human glioblastoma cells. The treatment with HeNPs induced dose and time dependent.
中枢神经系统肿瘤是成人中最常见的实体瘤。与其他类型的癌症不同，脑癌由于血脑屏障（BBB）而很难治疗，血脑屏障会阻止药物穿过并进入大脑。体内和体外已经使用了不同类型的克服血脑屏障的方法，其中使用纳米颗粒介导的治疗药物递送特别有前景。在本研究中，我们使用氧化铁磁性纳米颗粒（NPs）作为向日葵素（He/NPs）的载体系统来治疗源自胶质母细胞瘤的癌细胞。使用了在我们实验室中从一名被诊断患有胶质母细胞瘤的患者的组织中建立的早期传代细胞培养物（GB1B）。用不同浓度的NPs或HeNPs处理细胞，然后在24、48和72小时测量细胞增殖。我们的结果表明，胶质母细胞瘤细胞对NPs处理耐受性良好，处理后细胞活力略有增加。此外，我们证明了负载向日葵素的Fe3O4磁性纳米颗粒在人胶质母细胞瘤细胞中诱导细胞毒性。HeNPs处理诱导了剂量和时间依赖性。
